Mitogen-activated protein kinase/extracellular signal-regulated kinase 2 regulates cytoskeletal organization and chemotaxis via catalytic and microtubule-specific interactions
| Autor: | Alfred A. Reszka, J C Bulinski, E G Krebs, Edmond H. Fischer |
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| Rok vydání: | 1997 |
| Předmět: |
CHO Cells
Biology Microtubules Catalysis Feedback Focal adhesion Cricetinae Animals Cytoskeleton Molecular Biology Actin Adaptor Proteins Signal Transducing Cell Size GRB2 Adaptor Protein Mitogen-Activated Protein Kinase 1 Cell growth Chemotaxis Proteins Cell migration Cell Biology Cell biology Cytoskeletal Proteins Mitogen-activated protein kinase Calcium-Calmodulin-Dependent Protein Kinases biology.protein Mutagenesis Site-Directed Signal transduction Cell Division Signal Transduction Research Article |
| Zdroj: | Molecular biology of the cell. 8(7) |
| ISSN: | 1059-1524 |
| Popis: | The extracellular signal-regulated kinases (ERKs) 1 and 2 are mitogen-activated protein kinases that act as key components in a signaling cascade linking growth factor receptors to the cytoskeleton and the nucleus. ERK2 mutants have been used to alter cytoskeletal regulation in Chinese hamster ovary cells without affecting cell growth or feedback signaling. Mutation of the unique loop L6 (residues 91-95), which is in a portion of the molecule that is cryptic upon the binding of ERK2 to the microtubules (MTs), generated significant morphological alterations. Most notable phenotypes were observed after expression of a combined mutant incorporating changes to both L6 and the TEY phosphorylation lip, including a 70% increase in cell spreading. Actin stress fibers in these cells, which normally formed a single broad parallel array, were arranged in three or more orientations or in fan-like arrays. MTs, which ordinarily extend longitudinally from the centrosome, spread radially, covering a larger surface area. Single, but not the double, mutations of the Thr and Tyr residues of the TEY phosphorylation lip caused a ca. 25% increase in cell spreading, accompanied by a threefold increase in chemotactic cell migration. Mutation of Lys-52 triggered a 48% increase in cell spreading but no alteration to chemotaxis. These findings suggest that wild-type ERK2 inhibits the organization of the cytoskeleton, the spreading of the cell, and chemotactic migration. This involves control of the orientation of actin and MTs and the positioning of focal adhesions via regulatory interactions that may occur on the MTs. |
| Databáze: | OpenAIRE |
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