Novel dot-blot assay for detection of vascular Notch3 aggregates in patients with CADASIL
| Autor: | Yohei Misumi, Akihiko Ueda, Toshiya Nomura, Taro Yamashita, Akihito Nagatoshi, Yasuteru Inoue, Masayoshi Tasaki, Hirotaka Matsui, Yukio Ando, Teruaki Masuda, Masamitsu Okada, Mitsuharu Ueda, Satoru Shinriki, Aito Isoguchi, Yihong Ma |
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| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty Immunoblotting Dot blot CADASIL 03 medical and health sciences 0302 clinical medicine Biopsy Extracellular medicine Humans In patient 030212 general & internal medicine Receptor Notch3 Skin Receptors Notch biology medicine.diagnostic_test Chemistry medicine.disease Immunohistochemistry Neurology Mutation biology.protein Biopsy skin Neurology (clinical) Antibody 030217 neurology & neurosurgery |
| Zdroj: | Journal of the Neurological Sciences. 415:116931 |
| ISSN: | 0022-510X |
| DOI: | 10.1016/j.jns.2020.116931 |
| Popis: | To detect vascular Notch3 extracellular domain aggregates in CADASIL, we developed a novel dot-blot assay with both autopsy and biopsy skin samples. We obtained samples from 11 patients with CADASIL and 12 control patients, and we performed dot-blot analyses by using sequential biochemical tissue extractions with three different antibodies against specific regions of the Notch3 extracellular domain. We also analyzed clinical features and vascular accumulations of Notch3 by immunohistochemistry. Via the dot-blot assay with the antibody against the C-terminal region of the Notch3 extracellular domain, we successfully detected Notch3 extracellular domain aggregates in skin tissue homogenates obtained from patients with CADASIL. Our novel method may therefore aid the diagnosis of CADASIL. |
| Databáze: | OpenAIRE |
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