Enumeration of Escherichia coli in swab samples from pre- and post-chilled pork and lamb carcasses using 3M™ Petrifilm™ Select E. coli and Simplate® Coliforms/ E. coli
| Autor: | Sigrun J. Hauge, Truls Nesbakken, Marte Monshaugen, Ole Alvseike, Øyvin Østensvik, Ole-Johan Røtterud |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Swine animal diseases 030106 microbiology Colony Count Microbial Food Contamination Biology medicine.disease_cause Petrifilm Microbiology 03 medical and health sciences fluids and secretions 0404 agricultural biotechnology parasitic diseases Escherichia coli Enumeration medicine Animals Food science Pre and post Sheep Carcass contamination Temperature technology industry and agriculture food and beverages 04 agricultural and veterinary sciences 040401 food science Red Meat Concordance correlation coefficient Food Microbiology Abattoirs Food Science |
| Zdroj: | Meat Science. 130:26-29 |
| ISSN: | 0309-1740 |
| Popis: | The aim of the study was to compare two analytical methods; 3M Petrifilm™ Select E. coli and SimPlate® Coliforms &E. coli, for detection and enumeration of E. coli using swab samples from naturally contaminated pork and lamb carcasses that were collected before and after chilling. Blast chilling was used for pork carcasses. Swab samples (n=180) were collected from 60 warm and 60 chilled pork carcasses, and 30 warm and 30 chilled lamb carcasses, and analysed in parallel. The concordance correlation coefficient between Petrifilm and SimPlate was 0.89 for pork and 0.81 for lamb carcasses. However, the correlation was higher for warm carcasses (0.90) than chilled carcasses (0.72). For chilled lamb carcasses, the correlation was only 0.50, and SimPlate gave slightly higher results than Petrifilm (P=0.09). Slower chilling gave slightly lesser agreement between methods than for blast chilling, however, both Petrifilm and SimPlate methodologies are suitable and recommended for use in small laboratories in abattoirs. |
| Databáze: | OpenAIRE |
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