In vivo reflectance-mode confocal microscopy provides insights in human skin microcirculation and histomorphology
| Autor: | Andreas Gohritz, Peter M. Vogt, Matthias C. Aust, M.A. Altintas, Ahmet Ali Altintas, Max V. Meyer-Marcotty, Merlin Guggenheim |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Adolescent Capillary action Health Informatics Human skin Vasodilation Microcirculation law.invention Young Adult In vivo Confocal microscopy law medicine Humans Radiology Nuclear Medicine and imaging Skin Microscopy Confocal Radiological and Ultrasound Technology Chemistry Blood flow Computer Graphics and Computer-Aided Design Vasoconstriction Female Computer Vision and Pattern Recognition medicine.symptom Biomedical engineering |
| Zdroj: | Computerized medical imaging and graphics : the official journal of the Computerized Medical Imaging Society. 33(7) |
| ISSN: | 1879-0771 |
| Popis: | Purpose Various approaches are used to study microcirculation, however, no modality evaluates microcirculation and histomorphology on cellular levels. We hypothesized that reflectance-mode confocal microscopy (RCM) enables simultaneous evaluation in vivo of both microcirculation and histomorphology. Principals The forearm of 20 volunteers was exposed to either local heat stress (HS-group), or to local cold stress (CS-group). RCM was performed prior and after temperature stress to evaluate quantitative blood-cell flow, capillary loop diameter, granular cell size, and basal layer thickness. Results In the HS-group, we observed significant increase in capillary loop diameter and increased blood-cell flow after heat stress. In the CS-group, significant decreases of capillary loop diameter and in blood-cell flow were determined following cold stress. Granular cell size and basal layer thickness differed insignificantly prior and after local temperature stress. Conclusions RCM provides real-time and in vivo high resolution imaging of temperature-dependent changes in the human skin microcirculation and histomorphology on cellular levels. |
| Databáze: | OpenAIRE |
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