let-7i inhibits proliferation and migration of bladder cancer cells by targeting HMGA1
| Autor: | Zheng R, Pu C, Qin Mm, Gang Feng, Houbao Huang, Xue Chai, Liu Xc, Li Xn, Zhang J |
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| Rok vydání: | 2019 |
| Předmět: |
Urology
Proliferation 030232 urology & nephrology lcsh:RC870-923 urologic and male genital diseases 03 medical and health sciences 0302 clinical medicine Western blot Cell Movement Cell Line Tumor microRNA medicine Humans HMGA1a Protein Viability assay Migration Cell Line Transformed Cell Proliferation Let-7i High mobility group protein A1 Bladder cancer biology medicine.diagnostic_test Cell growth business.industry General Medicine Transfection lcsh:Diseases of the genitourinary system. Urology medicine.disease HMGA1 MicroRNAs Urinary Bladder Neoplasms Reproductive Medicine Cell culture 030220 oncology & carcinogenesis biology.protein Cancer research business Research Article |
| Zdroj: | BMC Urology BMC Urology, Vol 19, Iss 1, Pp 1-8 (2019) |
| ISSN: | 1471-2490 |
| DOI: | 10.1186/s12894-019-0485-1 |
| Popis: | Background Let-7 is one of the earliest discovered microRNAs(miRNAs) and has been reported to be down-regulated in multiple malignant tumors. The effects and molecular mechanisms of let-7i in bladder cancer are still unclear. This study was to investigate the effects and potential mechanisms of let-7i on bladder cancer cells. Methods Total RNA was extracted from bladder cancer cell lines. The expression levels of let-7i and HMGA1 were examined by quantitative real-time PCR. Cell viability was detected using the CCK-8 and colony formation assays, while transwell and wound healing assays were used to evaluate migration ability. Luciferase reporter assay and western blot were used to confirm the target gene of let-7i. Results Compared with the SV-40 immortalized human uroepithelial cell line (SV-HUC-1), bladder cancer cell lines T24 and 5637 had low levels of let-7i expression, but high levels of high mobility group protein A1 (HMGA1) expression. Transfection of cell lines T24 and 5637 with let-7i mimic suppressed cell proliferation and migration. Luciferase reporter assay confirmed HMGA1 may be one of the target genes of let-7i-5p. Protein and mRNA expression of HMGA1 was significantly downregulated in let-7i mimic transfected cell lines T24 and 5637. Conclusions Up-regulation of let-7i suppressed proliferation and migration of the human bladder cancer cell lines T24 and 5637 by targeting HMGA1. These findings suggest that let-7i might be considered as a novel therapeutic target for bladder cancer. |
| Databáze: | OpenAIRE |
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