Fast saccharide mapping method for quality consistency evaluation of commercial xylooligosaccharides collected in China
| Autor: | Ling-Xiao Chen, Cunwu Chen, Jing Zhao, Shao-Ping Li, Bangxing Han, Yong Deng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Resolution (mass spectrometry)
Pharmaceutical Science 02 engineering and technology Pharmacy RM1-950 Mass spectrometry 01 natural sciences High-performance liquid chromatography MALDI-MS Analytical Chemistry chemistry.chemical_compound Xylooligosaccharides Functional food Consistency (statistics) Health hazard Drug Discovery Electrochemistry Derivatization Spectroscopy Consistency analysis Chromatography Chemistry 010401 analytical chemistry Saccharide mapping Quality control 021001 nanoscience & nanotechnology 0104 chemical sciences Original Article Therapeutics. Pharmacology 0210 nano-technology |
| Zdroj: | Journal of Pharmaceutical Analysis, Vol 11, Iss 3, Pp 284-291 (2021) Journal of Pharmaceutical Analysis |
| ISSN: | 2095-1779 |
| Popis: | Due to the extensive use of xylooligosaccharides (XOS) as functional food ingredients, many inferior goods and even adulterants are generally found in the market, which may pose a health hazard to certain populations. Chromatography method such as high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) is traditionally applied for the quality analysis of XOS. However, it is time consuming due to the prolonged separation and pre- or post- derivatization procedure. In this study, a fast saccharide mapping method based on matrix-assisted laser desorption/time-of-flight mass spectrometry (MALDI-TOF-MS) was developed for the quality consistency analysis of 22 batches of XOS collected from different manufacturers in China. The time needed for saccharides analysis using MALDI-MS was less than 30 min for one plate, at least 6 times faster than that by the traditional HPTLC chromatography method. In addition, MALDI-MS possessed higher resolution for XOS with DP4-DP7 based on the difference of m/z, which is hardly separated using HPTLC. The results showed that XOS were present only in samples XY01-XY11, samples XY12-XY14 only consisted of hex oligosaccharides, and samples XY15-XY22 were free of oligosaccharides. These indicate that the quality consistency of XOS products in the China market was poor, which should be carefully investigated. Highlights • Fast saccharide mapping method was developed based on MADLI-TOF-MS. • Quality consistency of commercial xylooligosaccharides collected in China was evaluated. • Glycosidic linkage analysis was also used for identification of xylooligosaccharides. • Fifty percent of commercial xylooligosaccharides are mislabeled. |
| Databáze: | OpenAIRE |
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