The Vertebrate Protein Dead End Maintains Primordial Germ Cell Fate by Inhibiting Somatic Differentiation
| Autor: | Jan Bandemer, Theresa Gross-Thebing, Michal Reichman-Fried, Jochen Seggewiss, Mehdi Goudarzi, Christin Rathmer, Katsiaryna Tarbashevich, Jana Pfeiffer, Sargon Yigit, Erez Raz, Martin Stehling, Christian Ruckert |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
endocrine system Cell type Somatic cell Cellular differentiation Germ layer Biology Cell fate determination General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Cell Movement medicine Animals Cellular Reprogramming Techniques Molecular Biology In Situ Hybridization Zebrafish Endoderm RNA-Binding Proteins Cell Differentiation Cell Biology Zebrafish Proteins Cell biology Germ Cells 030104 developmental biology medicine.anatomical_structure Reprogramming 030217 neurology & neurosurgery Germ cell Developmental Biology |
| Zdroj: | Developmental Cell. 43:704-715.e5 |
| ISSN: | 1534-5807 |
| DOI: | 10.1016/j.devcel.2017.11.019 |
| Popis: | Maintaining cell fate relies on robust mechanisms that prevent the differentiation of specified cells into other cell types. This is especially critical during embryogenesis, when extensive cell proliferation, patterning, and migration events take place. Here we show that vertebrate primordial germ cells (PGCs) are protected from reprogramming into other cell types by the RNA-binding protein Dead end (Dnd). PGCs knocked down for Dnd lose their characteristic morphology and adopt various somatic cell fates. Concomitantly, they gain a gene expression profile reflecting differentiation into cells of different germ layers, in a process that we could direct by expression of specific cell-fate determinants. Importantly, we visualized these events within live zebrafish embryos, which provide temporal information regarding cell reprogramming. Our results shed light on the mechanisms controlling germ cell fate maintenance and are relevant for the formation of teratoma, a tumor class composed of cells from more than one germ layer. |
| Databáze: | OpenAIRE |
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