Effect of Salt Stress on Cytosine Methylation within GL2, An Arabidopsis thaliana Gene Involved in Root Epidermal Cell Differentiation. Absence of Inheritance in the Unstressed Progeny
| Autor: | Rodrigo Matías González, Cecilia Carmen Beyrne, Norberto Daniel Iusem |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine trichoblasts saline stress Root hair Arabidopsis SALINE STRESS 01 natural sciences Catalysis Inorganic Chemistry lcsh:Chemistry Ciencias Biológicas purl.org/becyt/ford/1 [https] 03 medical and health sciences GLABRA-2 Transcriptional regulation Arabidopsis thaliana root epidermis Epigenetics Physical and Theoretical Chemistry purl.org/becyt/ford/1.6 [https] Molecular Biology lcsh:QH301-705.5 DNA METHYLATION Spectroscopy DNA methylation biology TRICHOBLASTS Organic Chemistry Glabra-2 ROOT EPIDERMIS General Medicine Methylation Bioquímica y Biología Molecular biology.organism_classification ARABIDOPSIS Computer Science Applications Cell biology 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Root epidermal cell differentiation CIENCIAS NATURALES Y EXACTAS 010606 plant biology & botany |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET International Journal of Molecular Sciences Volume 20 Issue 18 International Journal of Molecular Sciences, Vol 20, Iss 18, p 4446 (2019) |
| Popis: | Methylation/demethylation of cytosines is an epigenetic strategy for transcriptional regulation, allowing organisms to rapidly respond and adapt to different stimuli. In this context, and using Arabidopsis thaliana as a plant model, we explored whether an environmental stress is sufficient to trigger a change in the methylation status of Glabra-2, a master gene associated with root epidermal cell differentiation. As this gene acts mainly in the epidermis in the root, we examined the stress-driven methylation levels specifically in that tissue. We focused on the stress caused by different salt concentrations in the growth medium. When testing the effect of 20 and 75 mM NaCl, we found that there is a significant decrease in the CG methylation level of the analyzed genomic region within the epidermis. Whereas this reduction was 23% in mildly stressed plants, it turned out to be more robust (33%) in severely stressed ones. Notably, this latter epigenetic change was accompanied by an increase in the number of trichoblasts, the epidermal cell type responsible for root hair development. Analysis of an eventual inheritance of epigenetic marks showed that the non-stressed progeny (F1) of stressed plants did not inherit&mdash in a Lamarckian fashion&mdash the methylation changes that had been acquired by the parental individuals. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|