Discordance in the Epithelial Cell-Dendritic Cell Major Histocompatibility Complex Class II Immunoproteome: Implications for Chlamydia Vaccine Development
| Autor: | Karuna P. Karunakaran, Robert C. Brunham, Queenie W.T. Chan, Raymond M. Johnson, Leonard J. Foster, Xiaozhou Jiang, Hong Yu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
CD4-Positive T-Lymphocytes
0301 basic medicine Chlamydia muridarum T cell 030106 microbiology Epitopes T-Lymphocyte Chlamydia trachomatis Major histocompatibility complex medicine.disease_cause Cell Line Mice Major Articles and Brief Reports 03 medical and health sciences Antigen MHC class I medicine Animals Humans Immunology and Allergy Antigens Bacterial MHC class II biology Histocompatibility Antigens Class I Histocompatibility Antigens Class II Epithelial Cells Dendritic Cells Dendritic cell Chlamydia Infections biology.organism_classification Mice Inbred C57BL 030104 developmental biology Infectious Diseases medicine.anatomical_structure Bacterial Vaccines Host-Pathogen Interactions Immunology biology.protein Female Peptides HeLa Cells |
| Zdroj: | J Infect Dis |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/jiz522 |
| Popis: | Background Chlamydia trachomatis and Chlamydia muridarum are intracellular bacterial pathogens of mucosal epithelial cells. CD4 T cells and major histocompatibility complex (MHC) class II molecules are essential for protective immunity against them. Antigens presented by dendritic cells (DCs) expand naive pathogen-specific T cells (inductive phase), whereas antigens presented by epithelial cells identify infected epithelial cells as targets during the effector phase. We previously showed that DCs infected by C trachomatis or C muridarum present epitopes from a limited spectrum of chlamydial proteins recognized by Chlamydia-specific CD4 T cells from immune mice. Methods We hypothesized that Chlamydia-infected DCs and epithelial cells present overlapping sets of Chlamydia-MHC class II epitopes to link inductive and effector phases to generate protective immunity. We tested that hypothesis by infecting an oviductal epithelial cell line with C muridarum, followed by immunoaffinity isolation and sequencing of MHC class I- and II-bound peptides. Results We identified 26 class I-bound and 4 class II-bound Chlamydia-derived peptides from infected epithelial cells. We were surprised to find that none of the epithelial cell class I- and class II-bound chlamydial peptides overlapped with peptides presented by DCs. Conclusions We suggest the discordance between the DC and epithelial cell immunoproteomes has implications for delayed clearance of Chlamydia and design of a Chlamydia vaccine. |
| Databáze: | OpenAIRE |
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