The Fabrication of Protein Nano Arrays Using 3-Dimensional Plastic Nanopillar Patterns
| Autor: | Soonmin Seo, Junhong Min, Ji Yeong Won, Jeong-Woo Choi |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Microscopy
Confocal Fabrication Materials science Ultraviolet Rays Protein Array Analysis Biomedical Engineering Proteins Bioengineering Nanotechnology General Chemistry Microscopy Atomic Force Condensed Matter Physics Soft lithography law.invention Solutions Confocal microscopy law Nano Microscopy Electron Scanning General Materials Science Protein pattern Lotus effect Biochip Nanopillar |
| Zdroj: | Journal of Nanoscience and Nanotechnology. 11:4231-4235 |
| ISSN: | 1533-4899 1533-4880 |
| DOI: | 10.1166/jnn.2011.3678 |
| Popis: | A plastic nanopillar array was used as the basis for development of a cheap, spatially patterned immobilization method that was applied to nano biochips. A plastic nanopillar array (diameter: 500 nm, height: 1.2 microm) was fabricated using poly(urethane acrylate) (PUA) by simple and fast UV-curable soft lithography. Antibodies were immobilized on top of the nanopillar structure due to the 'lotus effect'; the aqueous solution containing proteins could only contact the top portion of the nanopillar array due to the hydrophobicity of the surface. This phenomenon was verified by atomic force microscopy and confocal microscopy. Optimal conditions were investigated to effectively generate a clear protein pattern on the nanopillar array. The immunoreaction capability of captured antibodies immobilized on the nano pattern was validated using various concentrations of complimentary antibodies. |
| Databáze: | OpenAIRE |
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