Fluorescent aptasensor based on conformational switch–induced hybridization for facile detection of β-amyloid oligomers

Autor: Chun-Hsien Chen, Yuh-Jyh Jong, Yu-Ying Chao, Chun-Chi Wang, Yen-Ling Chen
Rok vydání: 2022
Předmět:
Zdroj: Analytical and Bioanalytical Chemistry. 414:8155-8165
ISSN: 1618-2650
1618-2642
DOI: 10.1007/s00216-022-04350-7
Popis: Aβ oligomers (AβO) are a dominant biomarker for early Alzheimer's disease diagnosis. A fluorescent aptasensor coupled with conformational switch-induced hybridization was established to detect AβO. The fluorescent aptasensor is based on the interaction of fluorophore-labeled AβO-specific aptamer (FAM-Apt) against its partly complementary DNA sequence on the surface of magnetic beads (cDNA-MBs). Once the FAM-Apt binds to AβO, the conformational switch of FAM-Apt increases the tendency to be captured by cDNA-MBs. This causes a descending fluorescence of supernatant, which can be utilized to determine the levels of AβO. Thus, the base-pair matching above 12 between FAM-Apt and cDNA-MBs with increasing hybridizing free energies reached the ascending fluorescent signal equilibrium. The optimized aptasensor showed linearity from 1.7 ng mL
Databáze: OpenAIRE
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