The discovery of a novel sodium channel in the cockroach Periplaneta americana: Evidence for an early duplication of the para-like gene

Autor: Christian Legros, Sophie Quinchard, Christophe Lemaire, Bruno Lapied, Bénédicte Moignot
Přispěvatelé: Laboratoire Récepteurs et Canaux Ioniques Membranaires (RCIM), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA), Pathologie Végétale (PaVé), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Laboratoire de Planétologie et Géodynamique UMR6112 (LPG), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Nantes - Faculté des Sciences et des Techniques, Université de Nantes (UN)-Université de Nantes (UN)-Université d'Angers (UA), Récepteurs et Canaux Ioniques Membranaires (RCIM), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Laboratoire de Planétologie et Géodynamique [UMR 6112] (LPG), Université d'Angers (UA)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2009
Předmět:
EXPRESSION DE GENE
MESH: Sequence Homology
Amino Acid
[SDV]Life Sciences [q-bio]
MOLECULAR CLONING
Cockroaches
MESH: Amino Acid Sequence
MESH: Protein Isoforms
CANAUX IONIQUES MEMBRANAIRES
Biochemistry
Sodium Channels
0302 clinical medicine
Gene Duplication
MESH: Insect Proteins
Protein Isoforms
MESH: Animals
ORFS
MESH: Phylogeny
Peptide sequence
Phylogeny
MESH: Evolution
Molecular
Genetics
0303 health sciences
biology
MESH: Gene Duplication
INSECTE
[SDE]Environmental Sciences
Insect Proteins
MESH: Cockroaches
Periplaneta
Molecular Sequence Data
Molecular cloning
MESH: Sodium Channels
Evolution
Molecular
03 medical and health sciences
MESH: Invertebrates
Complementary DNA
ION CHANNELS
Animals
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Amino Acid Sequence
Molecular Biology
Gene
Ion channel
030304 developmental biology
[SDV.GEN]Life Sciences [q-bio]/Genetics
MESH: Molecular Sequence Data
Sequence Homology
Amino Acid
Sodium channel
INSECT
biology.organism_classification
Invertebrates
Insect Science
030217 neurology & neurosurgery
Zdroj: Insect Biochemistry and Molecular Biology
Insect Biochemistry and Molecular Biology, Elsevier, 2009, 39 (11), pp.814-823. ⟨10.1016/j.ibmb.2009.09.006⟩
ISSN: 0965-1748
DOI: 10.1016/j.ibmb.2009.09.006⟩
Popis: International audience; Voltage-gated sodium channels (Na(v) channels) belong to a superfamily of ion channels which play an essential role in membrane excitability. Only one gene encoding Na(v) channels has been characterized so far in insects. Here, we have cloned one full-length cDNA encoding a conventional insect Na(v) channel (PaNa(v)1) and two full-length cDNAs encoding putative insect Na(v) channels (PaFPC1 and PaFPC2) in Periplaneta americana, a model insect for neurophysiological studies. The ORFs of PaFPC1 and PaFPC2 contained 4662 bp and encoded 1553 amino acid residues, and the ORF of PaNa(v)1 contained 6153 bp and encoded 2051 amino acid residues. PaFPC1 and PaFPC2 are two isoforms, which differ by eight single amino acid substitutions. PaFPC1 shares 37.5-55% protein identities with known insect Na(v) channels, while PaNa(v)1 shares 70-97.5% protein identities with these latter. Both PaFPC1 and PaFPC2 possess the molecular hallmarks of Na(v) channels except the motif involved in fast inactivation. Contrary to PaNa(v)1 transcripts which are expressed mainly in the central nervous system, those ones of PaFPC are also expressed in non-neuronal tissues (muscles, gut and mushroom-shaped accessory glands). A detailed phylogenetic analysis confirmed that PaNa(v)1 and PaFPC are evolutionarily closely related to insect Na(v) channel genes.
Databáze: OpenAIRE
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