The viability of lytic bacteriophage ΦD5 in potato-associated environments and its effect on Dickeya solani in potato (Solanum tuberosum L.) plants
| Autor: | Anna Smolarska, Zofia Ozymko, Robert Czajkowski |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Leaves Pectobacterium lcsh:Medicine Plant Science Bacteriophage Soil Vegetables Medicine and Health Sciences Bacteriophages lcsh:Science Pathogen education.field_of_study Multidisciplinary Virulence biology Plant Bacterial Pathogens Plant Anatomy food and beverages Plants Potting soil Equipment Sterilization Plant Tubers Horticulture Infectious Diseases Lytic cycle Viruses Potato Research Article Equipment Preparation Copper Sulfate Infectious Disease Control 030106 microbiology Population Plant Pathogens Solanum Research and Analysis Methods complex mixtures 03 medical and health sciences Filter Sterilization Botany education Lysogeny Plant Diseases Solanum tuberosum Tubers Plant Extracts Host (biology) lcsh:R fungi Organisms Biology and Life Sciences Plant Pathology biology.organism_classification 030104 developmental biology lcsh:Q Dickeya solani |
| Zdroj: | PLoS ONE PLoS ONE, Vol 12, Iss 8, p e0183200 (2017) |
| ISSN: | 1932-6203 |
| Popis: | Dickeya solani is one of the most important pectinolytic phytopathogens responsible for high losses in potato, especially in seed potato production in Europe. Lytic bacteriophages can affect the structure of the host population and may influence spread, survival and virulence of the pathogen and in consequence, infection of the plant. In this study, we aimed to acquire information on the viability of the broad host lytic bacteriophage ΦD5 on potato, as well as to apprehend the specific effect of this bacteriophage on its host D. solani type-strain in different settings, as a preliminary step to target co-adaptation of phages and host bacteria in plant environment. Viability of the ΦD5 phage in tuber extract, on tuber surface, in potting compost, in rainwater and on the leaf surface, as well as the effect of copper sulfate, were examined under laboratory conditions. Also, the interaction of ΦD5 with the target host D. solani in vitro and in compost-grown potato plants was evaluated. ΦD5 remained infectious in potato tuber extract and rain water for up to 72 h but was inactivated in solutions containing 50 mM of copper. The phage population was stable for up to 28 days on potato tuber surface and in potting compost. In both, tissue culture and compost-grown potato plants, ΦD5 reduced infection by D. solani by more than 50%. The implications of these findings are discussed. |
| Databáze: | OpenAIRE |
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