Soluble Cyanobacterial Carotenoprotein as a Robust Antioxidant Nanocarrier and Delivery Module
| Autor: | Svetlana S. Efimova, Alexey Stepanov, Olga S. Ostroumova, Alibek Abdrakhmanov, Nikolai N. Sluchanko, Thomas Friedrich, Pavel A. Babaev, Eugene G. Maksimov, Tatiana A. Slastnikova, Anastasia V. Ryabova, Ekaterina A. Slutskaya, Alexey V. Zamaraev, Yury B. Slonimskiy, Evgenia Yu. Parshina |
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| Rok vydání: | 2020 |
| Předmět: |
liposomes
0106 biological sciences 0301 basic medicine Antioxidant Physiology medicine.medical_treatment Clinical Biochemistry protein-protein interactions Antimycin A carotenoid delivery medicine.disease_cause 01 natural sciences Biochemistry Article 03 medical and health sciences chemistry.chemical_compound medicine oxidative stress Molecular Biology Carotenoid chemistry.chemical_classification Reactive oxygen species Liposome lcsh:RM1-950 food and beverages protein-membrane interactions Cell Biology carotenoid 540 Chemie und zugeordnete Wissenschaften lcsh:Therapeutics. Pharmacology 030104 developmental biology chemistry Echinenone ddc:540 Nanocarriers Oxidative stress 010606 plant biology & botany |
| Zdroj: | Antioxidants, Vol 9, Iss 869, p 869 (2020) Antioxidants Volume 9 Issue 9 |
| ISSN: | 2076-3921 |
| DOI: | 10.3390/antiox9090869 |
| Popis: | To counteract oxidative stress, antioxidants including carotenoids are highly promising, yet their exploitation is drastically limited by the poor bioavailability and fast photodestruction, whereas current delivery systems are far from being efficient. Here we demonstrate that the recently discovered nanometer-sized water-soluble carotenoprotein from Anabaena sp. PCC 7120 (termed AnaCTDH) transiently interacts with liposomes to efficiently extract carotenoids via carotenoid-mediated homodimerization, yielding violet&ndash purple protein samples. We characterize the spectroscopic properties of the obtained pigment&ndash protein complexes and the thermodynamics of liposome&ndash protein carotenoid transfer and demonstrate the delivery of carotenoid echinenone from AnaCTDH into liposomes with an efficiency of up to 70 ± 3%. Most importantly, we show efficient carotenoid delivery to membranes of mammalian cells, which provides protection from reactive oxygen species (ROS). Incubation of neuroblastoma cell line Tet21N in the presence of 1 &mu M AnaCTDH binding echinenone decreased antimycin A ROS production by 25% (p < 0.05). The described carotenoprotein may be considered as part of modular systems for the targeted antioxidant delivery. |
| Databáze: | OpenAIRE |
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