Emergence and genomic diversification of a virulent serogroup W:ST-2881(CC175) Neisseria meningitidis clone in the African meningitis belt
| Autor: | Katharina Röltgen, Ali Sié, Gerd Pluschke, Thomas Junghanss, Araceli Lamelas, Jean-Pierre Dangy, Abraham Hodgson, Stephen D. Bentley, Abdul-Wahab Mawuko Hamid, Simon R. Harris, Julia Hauser, Mohamad R. Abdul Sater, Julian Parkhill |
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| Přispěvatelé: | Parkhill, Julian [0000-0002-7069-5958], Apollo - University of Cambridge Repository |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
clone (Java method) Neisseria meningitidis serogroup W ST-2881 Gene Transfer Horizontal 030106 microbiology Virulence homologous recombination Biology Meningitis Meningococcal Neisseria meningitidis medicine.disease_cause emerging hypervirulent lineages Ghana Disease Outbreaks 03 medical and health sciences parasitic diseases Burkina Faso medicine Humans Colonization whole genome sequencing Communicable Disease Genomics Phylogenetic tree Outbreak General Medicine medicine.disease Virology 3. Good health African meningitis belt 030104 developmental biology Microbial Evolution and Epidemiology Meningitis Research Article |
| Zdroj: | Microbial Genomics |
| Popis: | Countries of the African 'meningitis belt' are susceptible to meningococcal meningitis outbreaks. While in the past major epidemics have been primarily caused by serogroup A meningococci, W strains are currently responsible for most of the cases. After an epidemic in Mecca in 2000, W:ST-11 strains have caused many outbreaks worldwide. An unrelated W:ST-2881 clone was described for the first time in 2002, with the first meningitis cases caused by these bacteria reported in 2003. Here we describe results of a comparative whole-genome analysis of 74 W:ST-2881 strains isolated within the framework of two longitudinal colonization and disease studies conducted in Ghana and Burkina Faso. Genomic data indicate that the W:ST-2881 clone has emerged from Y:ST-175(CC175) bacteria by capsule switching. The circulating W:ST-2881 populations were composed of a variety of closely related but distinct genomic variants with no systematic differences between colonization and disease isolates. Two distinct and geographically clustered phylogenetic clonal variants were identified in Burkina Faso and a third in Ghana. On the basis of the presence or absence of 17 recombination fragments, the Ghanaian variant could be differentiated into five clusters. All 25 Ghanaian disease isolates clustered together with 23 out of 40 Ghanaian isolates associated with carriage within one cluster, indicating that W:ST-2881 clusters differ in virulence. More than half of the genes affected by horizontal gene transfer encoded proteins of the 'cell envelope' and the 'transport/binding protein' categories, which indicates that exchange of non-capsular antigens plays an important role in immune evasion. |
| Databáze: | OpenAIRE |
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