Connection between the Gut Microbiome, Systemic Inflammation, Gut Permeability and FOXP3 Expression in Patients with Primary Sjögren’s Syndrome

Autor: Antonio Cano-Ortiz, Lidia Sánchez-Alcoholado, Marina Mora, Alberto Membrillo del Pozo, María Isabel Queipo-Ortuño, Isabel Leiva-Gea, Aurora Laborda-Illanes, Alberto Villarrubia Cuadrado, Isaac Plaza-Andrades
Rok vydání: 2020
Předmět:
0301 basic medicine
medicine.medical_treatment
Gut flora
Systemic inflammation
T-Lymphocytes
Regulatory
Body Mass Index
lcsh:Chemistry
Feces
RNA
Ribosomal
16S
Bacteroides
lcsh:QH301-705.5
Spectroscopy
biology
FOXP3
Forkhead Transcription Factors
General Medicine
Middle Aged
Interleukin-10
Computer Science Applications
Actinobacteria
Intestines
Sjogren's Syndrome
Cytokine
Female
medicine.symptom
Adult
Adolescent
primary Sjögren’s syndrome
030106 microbiology
Firmicutes
Inflammation
Article
Permeability
Catalysis
Proinflammatory cytokine
Inorganic Chemistry
03 medical and health sciences
Proteobacteria
medicine
Humans
Physical and Theoretical Chemistry
Molecular Biology
Aged
Intestinal permeability
gut microbiota
intestinal permeability
Organic Chemistry
Genetic Variation
biology.organism_classification
medicine.disease
Gastrointestinal Microbiome
stomatognathic diseases
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
inflammation
Case-Control Studies
Immunology
Dysbiosis
FOXP3 expression
Zdroj: International Journal of Molecular Sciences
Volume 21
Issue 22
International Journal of Molecular Sciences, Vol 21, Iss 8733, p 8733 (2020)
ISSN: 1422-0067
DOI: 10.3390/ijms21228733
Popis: The aims of this study were to explore intestinal microbial composition and functionality in primary Sjö
gren&rsquo
s syndrome (pSS) and to relate these findings to inflammation, permeability and the transcription factor Forkhead box protein P3 (FOXP3) gene expression in peripheral blood. The study included 19 pSS patients and 19 healthy controls matched for age, sex, and body mass index. Fecal bacterial DNA was extracted and analyzed by 16S rRNA sequencing using an Ion S5 platform followed by a bioinformatics analysis using Quantitative Insights into Microbial Ecology (QIIME II) and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). Our data suggest that the gut microbiota of pSS patients differs at both the taxonomic and functional levels with respect to healthy controls. The gut microbiota profile of our pSS patients was characterized by a lower diversity and richness and with Bacteroidetes dominating at the phylum level. The pSS patients had less beneficial or commensal butyrate-producing bacteria and a higher proportion of opportunistic pathogens with proinflammatory activity, which may impair intestinal barrier function and therefore contribute to inflammatory processes associated with pSS by increasing the production of proinflammatory cytokines and decreasing the release of the anti-inflammatory cytokine IL-10 and the peripheral FOXP3 mRNA expression, implicated in the development and function of regulatory T cells (Treg) cells. Further studies are needed to better understand the real impact of dysbiosis on the course of pSS and to conceive preventive or therapeutic strategies to counteract microbiome-driven inflammation.
Databáze: OpenAIRE
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