Prevalence of the GABAA receptor assemblies containing α1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: lack of modulation by chronic ethanol administration
| Autor: | Ashok K. Mehta, Maharaj K. Ticku |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
Azides medicine.medical_specialty Cerebellum Zolpidem Pyridines Immunoprecipitation Flunitrazepam Biology Tritium Rats Sprague-Dawley Benzodiazepines Radioligand Assay Cellular and Molecular Neuroscience chemistry.chemical_compound Internal medicine medicine Animals Hypnotics and Sedatives GABA Modulators GABA Agonists Molecular Biology Brain Chemistry Cerebral Cortex Ethanol Muscimol GABAA receptor musculoskeletal neural and ocular physiology Central Nervous System Depressants Affinity Labels Receptors GABA-A Precipitin Tests Rats Alcoholism medicine.anatomical_structure Endocrinology chemistry Cerebral cortex Chronic Disease psychological phenomena and processes medicine.drug |
| Zdroj: | Molecular Brain Research. 67:194-199 |
| ISSN: | 0169-328X |
| DOI: | 10.1016/s0169-328x(99)00020-0 |
| Popis: | The anti-alpha1 antibody elicited higher immunoprecipitation (%) values of the [3H]flunitrazepam and [3H]muscimol binding activity in the rat cerebellum vs. cerebral cortex, whereas immunoprecipitation values for [3H]Ro 15-4513 and [3H]zolpidem were comparable in these brain regions. Chronic ethanol administration neither changed the radioligand binding to the immunoprecipitated pellet nor the percentage immunoprecip-itation values, thereby indicating that chronic ethanol did not result in down-regulation of the GABAA receptor assemblies containing alpha1-subunit. |
| Databáze: | OpenAIRE |
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