Effect of acupuncture at Renying (ST 9) on gene expression profile of hypothalamus in spontaneously hypertensive rats
| Autor: | Zhu Shipeng, Zhang Wei, Guo Yan, Ma Liangxiao, Lu Juan, Park Kibeum, Liang Jingrong, Zhao Ruili, Xu Jing, Chen Huan |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
medicine.medical_specialty
Vascular smooth muscle business.industry General Medicine 030204 cardiovascular system & hematology Essential hypertension medicine.disease 03 medical and health sciences 0302 clinical medicine Endocrinology Real-time polymerase chain reaction Hypothalamus Internal medicine Gene expression Acupuncture medicine Gene chip analysis business Gene 030217 neurology & neurosurgery |
| Zdroj: | Journal of Traditional Chinese Medicine. 38:227-241 |
| ISSN: | 0254-6272 |
| DOI: | 10.1016/j.jtcm.2018.04.008 |
| Popis: | OBJECTIVE To investigate changes in gene expression profiles in the hypothalamus related to the effects of acupuncture at the Renying (ST 9) acupoint in spontaneously hypertensive (SH) rats. METHODS We randomly divided 18 SH rats into Renying (ST 9) group and model control group, 9 body weight-matched Wistar-Kyoto rats were used as blank controls. Acupuncture was performed manually for 20-min daily over 28 d in the Renying (ST 9) group. Rat Gene 2.0 array technology was used for the determination of gene expression profiles and the screened key genes were verified by real-time quantitative polymerase chain reaction (RT-PCR) analyses. RESULTS The different groups exhibited differential gene expression: compared with the blank control group, 48 genes were up-regulated and 91 genes were down-regulated in the model group; compared with the model group, 79 genes were up-regulated and 80 genes were down-regulated in Renying (ST 9) group. The RT-PCR results of the key genes including Chi3l1, Ephx2, Klk1, 5-HT1A and Cbs were consistent with that of gene chip analysis. CONCLUTION Acupuncture at Renying (ST 9) could significantly lower the blood pressure of SH rats and affect their hypothalamic gene expression profile. Genes associated with the contraction of vascular smooth muscle and the regulation of inflammation, neurotransmitters may be involved in acupuncture's antihypertensive mechanism. |
| Databáze: | OpenAIRE |
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