Expression of Receptors for Glial Cell Line-derived Neurotrophic Factor (GDNF) and Neurturin in the Inner Blood-retinal Barrier of Rats
| Autor: | Norimasa Sawada, Hirotoshi Tobioka, Tsutomu Ishizaki, Masaaki Satoh, Hideaki Miyajima, Kazuhide Kuwahara, Hideki Chiba, Hiroyuki Utsumi, Tomoko Gotoh, Michio Mori, Yo Igarashi |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Glial Cell Line-Derived Neurotrophic Factor Receptors Swine Physiology animal diseases Neurturin Blood–retinal barrier Receptors Nerve Growth Factor Rats Sprague-Dawley Neurotrophic factors Proto-Oncogene Proteins Blood-Retinal Barrier Electric Impedance medicine Glial cell line-derived neurotrophic factor Animals Drosophila Proteins Nerve Growth Factors Molecular Biology Cells Cultured Cerebral Cortex Retina Glial fibrillary acidic protein biology urogenital system Chemistry Proto-Oncogene Proteins c-ret Receptor Protein-Tyrosine Kinases Cell Biology General Medicine Anatomy Immunohistochemistry Rats Cell biology medicine.anatomical_structure nervous system Astrocytes biology.protein Cattle Endothelium Vascular sense organs GDNF family of ligands |
| Zdroj: | Cell Structure and Function. 25:237-241 |
| ISSN: | 1347-3700 0386-7196 |
| DOI: | 10.1247/csf.25.237 |
| Popis: | The retina is protected from somatic circulation by the blood-retinal barrrier (BRB) composed of tight junctions between retinal vascular endothelial cells (the inner BRB) and those between retinal pigment epithelial cells (the outer BRB). Our recent studies showed that glial cell line-derived neurotrophic factor (GDNF) secreted from astrocytes regulates the permeability of the BBB. In the present study, we immunohistochemically examined the expression of GDNF, neurturin (NTN) and their receptors, GFRalpha1 for GDNF and GFRalpha2 for NTN, because the capillaries of the inner BRB show specialization very similar to the blood-brain barrier (BBB). GDNF and NTN were detected in glial fibrillary acidic protein (GFAP)-positive cells, including Müller cells. GFRalpha1 and GFRalpha2 were localized in von Willebrand factor-positive cells. GDNF and NTN enhanced the barrier function of endothelial cells derived from porcine brain cortex. These results strongly suggest that the barrier function of the BRB is regulated by GDNF and NTN secreted from glial cells, like the BBB. |
| Databáze: | OpenAIRE |
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