Aerobic exercise training partially reverses the impairment of Nrf2 activation in older humans
| Autor: | Ethan L. Ostrom, Tinna Traustadóttir |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty NF-E2-Related Factor 2 Biochemistry Peripheral blood mononuclear cell digestive system environment and public health Antioxidants Article law.invention 03 medical and health sciences Basal (phylogenetics) 0302 clinical medicine Randomized controlled trial law Physiology (medical) Internal medicine Gene expression Aerobic exercise Medicine Humans Transcription factor Exercise Balance (ability) Aged business.industry respiratory system 030104 developmental biology Endocrinology GCLC Gene Expression Regulation Female business 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Free Radic Biol Med |
| ISSN: | 1873-4596 |
| Popis: | Nuclear factor erythroid-2-related factor 2 (Nrf2), is an inducible transcription factor that improves redox balance through stimulating antioxidant gene expression. In older humans the Nrf2 response to a single bout of acute exercise is blunted compared to young indicating impaired redox signaling. The purpose of this randomized controlled trial was to investigate if the signaling impairment could be reversed with exercise training in older men and women, while also comparing to young. Young (18–28y, n=21) and older (≥60y, n=19) men and women were randomized to 8-week aerobic exercise training (ET; 3d/wk, 45min/d) or a non-exercise control group (CON). Nrf2 nuclear localization, gene expression for NQO1, HO1, and GCLC, and GCLC protein were measured in PBMCs in response to acute exercise trial (AET; 30-min cycling at 70% VO(2) peak pre- and post-intervention at 7 timepoints (Pre, +10m, +30m, +1h, +4h, +8h, +24h). Young had greater Nrf2 signaling response compared to older at pre-intervention (p=0.05), whereas the older had significantly higher basal Nrf2 levels (p=0.004). ET decreased basal Nrf2 expression compared to CON (p=0.032) and improved the Nrf2 signaling response in both young and older (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect