Detection of bcr-abl gene expression at a low level in blood cells of some patients with essential thrombocythemia
| Autor: | Ming-Der Liu, Hui-Chi Hsu, Yuan-Ming Lee, Wen-Hui Tsai, Chien-Hui Lieu, Chi-Kuan Ho, Jie-Yu You, Lo-Chun Au, Lin-Ya Tan |
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| Rok vydání: | 2004 |
| Předmět: |
Genetic Markers
Male Transcription Genetic Fusion Proteins bcr-abl Biology Genes abl Philadelphia chromosome Pathology and Forensic Medicine Fusion gene Leukocyte Count Polycythemia vera Reference Values hemic and lymphatic diseases Leukemia Myelogenous Chronic BCR-ABL Positive medicine Humans Lymphocytes neoplasms Aged ABL Myeloproliferative Disorders Essential thrombocythemia Myeloid leukemia General Medicine Middle Aged medicine.disease Molecular biology Exact test Gene Expression Regulation Immunology Female Chronic myelogenous leukemia Thrombocythemia Essential |
| Zdroj: | The Journal of laboratory and clinical medicine. 143(2) |
| ISSN: | 0022-2143 |
| Popis: | The major bcr-abl fusion gene is seen as a major marker of chronic myeloid leukemia (CML). However, whether the bcr-abl transcript can be detected in patients with essential thrombocythemia (ET) is still a matter of controversy. We detected the messenger RNA expression of the bcr-abl gene using reverse transcription–polymerase chain reaction in peripheral-blood leukocytes (PBLs) from 63 patients with myeloproliferative disorders (including CML, ET, and polycythemia vera [PV]) and 51 normal, healthy volunteers. The bcr-abl transcript was detected in 4 of the 30 ET patients (13.3%), 17 of the 17 CML patients (100%), none of the 16 PV patients (0%), and 1 of the 51 normal subjects (1.9%). Compared with the normal controls, ET patients have a greater tendency to express the bcr-abl transcript in PBLs ( P = .06, Fisher's exact test). Further semiquantitative analysis showed that the intensity of bcr-abl transcript expression in 4 ET patients and a normal individual was 10 3 to 10 4 times less than that in the CML patients. We conclude that the bcr-abl transcript can be detected in the PBLs of Philadelphia chromosome (Ph)-negative ET patients but that the level of expression is markedly less than that in CML patients. The clinical significance of this finding merits further investigation. |
| Databáze: | OpenAIRE |
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