Stage‐Specific Adaptation of HypermutablePseudomonas aeruginosaIsolates during Chronic Pulmonary Infection in Patients with Cystic Fibrosis
| Autor: | Sabine Schmoldt, Jürgen Heesemann, Michael Hogardt, Lutz Bader, Christina Hoboth, Christine Henke |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Lung Diseases Cystic Fibrosis DNA Repair Virulence Biology medicine.disease_cause Cystic fibrosis Microbiology Drug Resistance Multiple Bacterial Genotype medicine Humans Immunology and Allergy Pseudomonas Infections Gene Inflammation Lung Pseudomonas aeruginosa medicine.disease Phenotype Anti-Bacterial Agents Infectious Diseases medicine.anatomical_structure Chronic Disease Immunology DNA mismatch repair |
| Zdroj: | The Journal of Infectious Diseases. 195:70-80 |
| ISSN: | 1537-6613 0022-1899 |
| Popis: | Background. Pseudomonas aeruginosa (PA) strains with defective DNA mismatch repair genes generate numerous bacterial variants because of high mutation rates. In cystic fibrosis (CF), such mutator strains may lead to the rapid selection of survivors that are specifically adapted to the hostile environment of the inflamed CF lung. Methods. Genotypes and phenotypes of 111 PA variants descending from 3 distinct mutator strains obtained from 3 patients with CF were systematically characterized. Results. We demonstrated that PA mutS isolates accumulated in the CF lung during the observation period of 3-6 years, with dominance during the final stage of the disease. Mutator strains from the final stage of disease were multiresistant and had lost a set of established virulence-associated traits, including cytotoxicity for bronchial epithelial cells (Calu-3) and macrophages (J774). This pathoadaptation was associated with the loss of survival capacity in a typical environmental habitat, such as tap water. Strikingly, nonmutator strains that maintained their virulence potential persisted as a minority, probably with a preference for the lower airways. Conclusions. Mutator strains may evolve from the initially infecting PA strain and generate numerous variants with a loss of destructive virulence factors, probably because of selection for improved survival in the deteriorated CF lung but at the expense of the ability to live freely. |
| Databáze: | OpenAIRE |
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