Internalization of human interleukin 4 and transient down-regulation of its receptor in the CD23-inducible Jijoye cells
| Autor: | Helene Cabrillat, C E Zuber, Jean-Pierre Galizzi, Jacques Banchereau, Odile Djossou |
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| Rok vydání: | 1989 |
| Předmět: |
Azides
Time Factors Transcription Genetic Cytochalasin B media_common.quotation_subject Receptors Fc Cycloheximide Biology In Vitro Techniques Endocytosis Biochemistry Cell Line chemistry.chemical_compound Cell surface receptor Humans Internalization Receptor Molecular Biology Interleukin 4 media_common Receptors IgE Interleukins CD23 Temperature Chloroquine Cell Biology Virology Molecular biology Receptors Interleukin-4 Antigens Differentiation B-Lymphocyte Kinetics chemistry Gene Expression Regulation Cell culture Receptors Mitogen Interleukin-4 Lysosomes |
| Zdroj: | The Journal of biological chemistry. 264(12) |
| ISSN: | 0021-9258 |
| Popis: | Human interleukin 4 (IL-4) specifically induces the low affinity receptor for IgE (Fc epsilon R2/CD23) on the surface of the Burkitt lymphoma cell line Jijoye. At 4 degrees C 125I-IL-4 specifically binds to high affinity receptors (Kd = 4-10 x 10(-11) M; Bmax, 600-1,200 sites/cell). Following a rapid temperature shift from 4 to 37 degrees C, 80% of the receptor-bound 125I-IL-4 disappeared from the cell surface within 20 min (t1/2 = 8.9 min). For every two internalized molecules of IL-4 (t1/2 = 13 min), one molecule of IL-4 dissociated from the cell surface (t1/2 = 25 min). More than 90% of the internalized IL-4 was released in a degraded form into the medium following first order kinetics (t1/2 = 68 min). Internalization was inhibited by cytoskeletal disrupting and lysosomotropic agents. Incubating cells with 1 nM IL-4 resulted in a rapid down-regulation of IL-4 receptors (75% loss after 2 h); a reexpression of receptor to control level occurred after 20 h in spite of the presence of a large excess of IL-4. Reexpression was delayed by chloroquine and blocked by cycloheximide and actinomycin D. By using the cross-linking agent bis(sulfosuccinimidyl)suberate, three polypeptides of Mr 130,000, 80,000, and 70,000 were specifically labeled with 125I-IL-4. These three polypeptides coordinately disappeared and reappeared with the 125I-IL-4 binding sites. The induction of Fc epsilon R2/CD23 required prolonged incubation (greater than 8 h) with IL-4 and thus may be dependent on the reexpression of IL-4 receptor. |
| Databáze: | OpenAIRE |
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