GATA4 is upregulated in nasopharyngeal cancer and facilitates epithelial‑mesenchymal transition and metastasis through regulation of SLUG
| Autor: | Beibei Yang, Huiyu Chang, Yibin Zhou |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
endocrine system Cancer Research Cell Biology Metastasis 03 medical and health sciences 0302 clinical medicine Immunology and Microbiology (miscellaneous) medicine Epithelial–mesenchymal transition reproductive and urinary physiology Oncogene Cell growth Mesenchymal stem cell Cancer Articles General Medicine respiratory system Cell cycle medicine.disease 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis embryonic structures cardiovascular system Cancer research |
| Zdroj: | Experimental and Therapeutic Medicine. |
| ISSN: | 1792-1015 1792-0981 |
| DOI: | 10.3892/etm.2018.6826 |
| Popis: | GATA4, a member of the GATA family, serves a key function in several types of cancer, including hepatoblastoma, gastric cancer and breast cancer. However, the function of GATA4 in nasopharyngeal cancer (NPC) is largely unknown. The present study revealed that GATA4 was upregulated in NPC tissue samples and the NPC cell line, 5–8F. Furthermore, the expression of GATA4 was associated with tumor size, metastasis and poor prognosis. Transwell invasion and wound healing analyses demonstrated that GATA4 promoted cell invasion and migration, respectively. Western blotting and reverse transcription-quantitative polymerase chain reaction revealed that GATA4 overexpression decreased the expression of epithelial markers and increased the expression of mesenchymal markers. By contrast, GATA4 inhibition increased the expression of epithelial markers and decreased the mesenchymal markers. Additionally, chromatin immunoprecipitation and dual-luciferase reporter assays revealed that GATA4 promoted epithelial-mesenchymal transition through transcriptionally activating SLUG. Cell counting kit-8 and colony formation assays were performed to analyze the effect of GATA4 on cell proliferation. The results indicated that GATA4 facilitated cell proliferation in NPC. In conclusion, GATA4 acts as an oncogene and serves crucial roles in NPC and GATA4 may find a potential application as therapeutic option in NPC. |
| Databáze: | OpenAIRE |
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