Improving spectral resolution in biological solid-state NMR using phase-alternated rCW heteronuclear decoupling
| Autor: | Morten Bjerring, Niels Chr. Nielsen, Asif Equbal, Perunthiruthy K. Madhu |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
SPECTROSCOPY
Spins MEMBRANE-PROTEINS Chemistry ASSIGNMENT Analytical chemistry General Physics and Astronomy Decoupling (cosmology) FREQUENCY Homonuclear molecule Spectral line ANGLE-SPINNING NMR AMYLOID FIBRILS Solid-state nuclear magnetic resonance Heteronuclear molecule Chemical physics ROTATING SOLIDS SIMULATION RF IRRADIATION NUCLEAR-MAGNETIC-RESONANCE Physical and Theoretical Chemistry Spectral resolution Magnetic dipole–dipole interaction |
| Zdroj: | Equbal, A, Bjerring, M, Madhu, P K & Nielsen, N C 2015, ' Improving spectral resolution in biological solid-state NMR using phase-alternated rCW heteronuclear decoupling ', Chemical Physics Letters, vol. 635, pp. 339-344 . https://doi.org/10.1016/j.cplett.2015.07.008 |
| ISSN: | 0009-2614 |
| Popis: | The successful application of solid-state NMR spectroscopy for structural study of biological macromolecules requires high spectral resolution. In presence of abundant H 1 spins, the resolution of the prevailing C 13 or N 15 chemical shift encoding experiments critically depends on the availability of efficient and robust heteronuclear decoupling methods in addition to the use of high-field instrumentation and fast sample spinning. Robustness of the decoupling method towards alterations in amplitude/offset of radio frequency fields due to varying sample states is important to ensure recording of spectra with high resolution over long sampling periods for insensitive samples. Here, we present a phase-alternated refocused continuous-wave decoupling method offering better resolution, easier setup, and higher robustness than previous methods. Improved decoupling is in part ascribed to more efficient cancellation of the residual heteronuclear, H 1 – C 13 , dipolar coupling interactions which are induced by homonuclear, H 1 – H 1 , dipolar coupling interactions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect