Biological Activities of Selected Plants and Detection of Bioactive Compounds from Ardisia elliptica Using UHPLC-Q-Exactive Orbitrap Mass Spectrometry
| Autor: | Faridah Abas, Siti Norhamimah Mohamed Yunus, Nurul Azila Fauzi, Awanis Azizan, Siti Zulaikha Abd Ghafar, Pei Lou Wong, Nur Ashikin Abdul Hamid, Nur Khaleeda Zulaikha Zolkeflee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
antioxidant
DPPH Ardisia elliptica Pharmaceutical Science Spondias 01 natural sciences Analytical Chemistry Cynometra cauliflora lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound lcsh:Organic chemistry Drug Discovery Gallic acid underutilized plants anti-α-glucosidase Physical and Theoretical Chemistry music 030304 developmental biology Ficus auriculata 0303 health sciences music.instrument biology Traditional medicine 010401 analytical chemistry Organic Chemistry fungi food and beverages Annona squamosa free radical scavenging biology.organism_classification 0104 chemical sciences phytochemical characterization chemistry Chemistry (miscellaneous) Molecular Medicine Quercetin |
| Zdroj: | Molecules Volume 25 Issue 13 Molecules, Vol 25, Iss 3067, p 3067 (2020) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules25133067 |
| Popis: | Plants and plant-based products have been used for a long time for medicinal purposes. This study aimed to determine the antioxidant and anti-&alpha glucosidase activities of eight selected underutilized plants in Malaysia: Leucaena leucocephala, Muntingia calabura, Spondias dulcis, Annona squamosa, Ardisia elliptica, Cynometra cauliflora, Ficus auriculata, and Averrhoa bilimbi. This study showed that the 70% ethanolic extract of all plants exhibited total phenolic content (TPC) ranging from 51 to 344 mg gallic acid equivalent (GAE)/g dry weight. A. elliptica showed strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide (NO) scavenging activities, with half maximal inhibitory concentration (IC50) values of 2.17 and 49.43 &mu g/mL, respectively. Most of the tested plant extracts showed higher inhibition of &alpha glucosidase enzyme activity than the standard, quercetin, particularly A. elliptica, F. auriculata, and M. calabura extracts with IC50 values of 0.29, 0.36, and 0.51 &mu g/mL, respectively. A total of 62 metabolites including flavonoids, triterpenoids, benzoquinones, and fatty acids were tentatively identified in the most active plant, i.e., A. elliptica leaf extract, by using ultra-high-performance liquid chromatography (UHPLC)&ndash electrospray ionization (ESI) Orbitrap MS. This study suggests a potential natural source of antioxidant and &alpha glucosidase inhibitors from A. elliptica. |
| Databáze: | OpenAIRE |
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