Human Papillomavirus Testing in Women With Mild Cytologic Atypia
| Autor: | Bergeron, Christine, Jeannel, Dominique, Poveda, Jean-Dominique, Cassonnet, Patricia, Orth, Gérard |
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| Přispěvatelé: | Laboratoire Pasteur Cerba, Institut Pasteur [Paris]-Laboratoire CERBA [Saint Ouen l'Aumône], Epidémiologie et Physiopathologie des Virus Oncogènes (EPVO (UMR_3569 / U-Pasteur_3)), Institut Pasteur [Paris]-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Papillomavirus, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Laboratoire CERBA [Saint Ouen l'Aumône], Institut Pasteur [Paris] (IP)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Rok vydání: | 2000 |
| Předmět: |
MESH: Vaginal Smears
[SDV]Life Sciences [q-bio] Uterine Cervical Neoplasms Polymerase Chain Reaction Sensitivity and Specificity 03 medical and health sciences 0302 clinical medicine MESH: Papillomaviridae MESH: Blotting Southern Humans 030212 general & internal medicine Papillomaviridae Vaginal Smears MESH: Humans virus diseases Obstetrics and Gynecology MESH: Polymerase Chain Reaction RNA Probes Uterine Cervical Dysplasia female genital diseases and pregnancy complications MESH: Sensitivity and Specificity MESH: DNA Viral 3. Good health MESH: Uterine Cervical Neoplasms Blotting Southern Colposcopy 030220 oncology & carcinogenesis DNA Viral MESH: RNA Probes MESH: Colposcopy Female MESH: Cervical Intraepithelial Neoplasia MESH: Female |
| Zdroj: | Clinical and experimental obstetrics & gynecology Clinical and experimental obstetrics & gynecology, Irog Canada Inc., 2000, 95 (6), pp.821-827. ⟨10.1016/s0029-7844(00)00795-x⟩ Clinical and experimental obstetrics & gynecology, 2000, 95 (6), pp.821-827. ⟨10.1016/s0029-7844(00)00795-x⟩ |
| ISSN: | 0029-7844 0390-6663 |
| DOI: | 10.1097/00006250-200006000-00008 |
| Popis: | International audience; Objective: To evaluate the efficiency of human papillomavirus (HPV) testing by Hybrid Capture II (Digene Diagnostics Inc., Silver Spring, MD) with regard to detecting biopsy-confirmed cervical intraepithelial neoplasia (CIN) or high-grade CIN in women with mild atypia, compared with the efficiencies of polymerase chain reaction (PCR), Southern blot hybridization, and cytology.Methods: We prospectively studied 378 women with atypical squamous cells of undetermined significance (ASCUS) (n = 111) or low-grade squamous intraepithelial lesions (SILs) (n = 267) demonstrated by referral cytology. We did repeat cytology, sampling for detection of HPV DNA by Hybrid Capture II, PCR, and Southern blot hybridization, and colposcopic evaluation with cervical biopsies.Results: All participants underwent the Hybrid Capture II test and 320 underwent the three HPV tests. Sensitivities of Hybrid Capture II for detecting CIN and high-grade CIN (0.81 and 0.86, respectively) were similar to those of cytology (0.83 and 0.82, respectively) and PCR (0.77 and 0.95, respectively), and higher than those of Southern blot hybridization (0.48 and 0.45, respectively). Compared with cytology, combined triage with Hybrid Capture II improved sensitivities for detecting CIN (0.94 versus 0.83, P < .001) and high-grade CIN (0.96 versus 0.85), though the latter difference was not significant (P = .17). In women with ASCUS, sensitivities of combined triage and cytology for detecting CIN were 0.94 and 0.71, respectively (P = .01), and sensitivities of the two methods for detecting high-grade CIN were 0.92 and 0.66, respectively (P = .13). The increase in sensitivity was lower among women with low-grade SILs; for these women, cytology had high sensitivity (0.86 for CIN and 1.00 for high-grade CIN). The specificity of combined triage was significantly lower than that of cytology in both groups.Conclusion: Compared with repeat cytology, combined triage with HPV testing markedly improves sensitivity for detecting CIN in women with ASCUS, but at the expense of specificity. |
| Databáze: | OpenAIRE |
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