| Autor: |
Shubha, P., Namratha, K., Gowda, M. Likhith, Nayan, Mb., Manjunatha, Hb., Byrappa, K. |
| Rok vydání: |
2022 |
| Předmět: |
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| DOI: |
10.6084/m9.figshare.20229927.v1 |
| Popis: |
The present study aims at the evaluation of in vitro and in vivo toxicities of green TiO2 nanoparticles (GS-TiO2) that are biosynthesised using hydroalcoholic extract of Ocimum sanctum leaves using microwave energy. Commercially available TiO2 NPs (CS-TiO2) were used for comparison. In vitro hemotoxicity was evaluated using haemolysis assay, and viability of fibroblast cells using WST-8 assay. In vivo toxicity evaluation was performed using the silkworm model. Haemolysis assay showed that GS-TiO2 caused acceptable haemolysis of 2.3% and CS-TiO2 showed higher haemolysis of 4.4%. As per WST-8 assay, viability of fibroblast cells was better in groups treated with GS-TiO2 with more than 50% survival. Silkworm growth parameters were significantly improved in GS-TiO2-treated batches. At the biomolecular level, changes in haemolymph protein banding patterns indicated strong growth promoting potential of GS-TiO2. Considering the salient findings derived from toxicity results, we propose that GS-TiO2 shall be potent NPs for various biomedical applications. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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