Detection and identification of mycobacteria by amplification of rRNA
| Autor: | B. Böddinghaus, Till Rogall, Erik C. Böttger, H. Blöcker, T. Flohr |
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| Rok vydání: | 1990 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Transcription Genetic Molecular Sequence Data Mycobacterium genavense Paratuberculosis Polymerase Chain Reaction Sensitivity and Specificity Mycobacterium Microbiology law.invention Mycobacterium tuberculosis Species Specificity law RNA Ribosomal 16S medicine Polymerase chain reaction Base Sequence biology Oligonucleotide Gene Amplification Ribosomal RNA medicine.disease biology.organism_classification RNA Ribosomal Oligonucleotide Probes Bacteria Research Article |
| Zdroj: | Journal of Clinical Microbiology. 28:1751-1759 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.28.8.1751-1759.1990 |
| Popis: | Oligonucleotides specific at a genus, group, or species level were defined by a systematic comparison of small-subunit rRNA sequences from Mycobacterium tuberculosis, M. bovis, M. africanum, M. bovis BCG, M. avium, M. kansasii, M. marinum, M. gastri, M. chelonae, M. smegmatis, M. terrae, M. nonchromogenicum, M. xenopi, M. malmoense, M. szulgai, M. scrofulaceum, M. fortuitum, M. gordonae, M. intracellulare, M. simiae, M. flavescens, M. paratuberculosis, M. sphagni, M. cookii, M. komossense, M. phlei, and M. farcinica. On the basis of the defined oligonucleotides, the polymerase chain reaction technique was explored to develop a sensitive taxon-specific detection system for mycobacteria. By using M. tuberculosis as a model system, fewer than 10 bacteria could be reliably detected by this kind of assay. These results suggest that amplification of rRNA sequences by the polymerase chain reaction may provide a highly sensitive and specific tool for the direct detection of microorganisms without the need for prior cultivation. |
| Databáze: | OpenAIRE |
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