Nitrogen oxidation consortia dynamics influence the performance of full-scale rotating biological contactors
| Autor: | Bruce Jefferson, D. Freeman, Boyd A. McKew, Dave R. Clark, Corinne Whitby, Francis Hassard, Frederic Coulon, Y. Bajón Fernández, A. Wilson |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
010504 meteorology & atmospheric sciences
Nitrogen Microorganism Wastewater treatment 010501 environmental sciences Rotating biological contactor 01 natural sciences Ammonia RNA Ribosomal 16S Ammonia oxidising bacteria Nitrogen cycle lcsh:Environmental sciences Soil Microbiology Ammonia oxidising archaea 0105 earth and related environmental sciences General Environmental Science lcsh:GE1-350 biology Chemistry Biofilm hemic and immune systems biology.organism_classification Archaea Nitrification Microbial population biology Environmental chemistry Oxidation-Reduction Bacteria circulatory and respiratory physiology |
| Zdroj: | Environment International, Vol 135, Iss, Pp-(2020) |
| ISSN: | 0160-4120 |
| DOI: | 10.1016/j.envint.2019.105354 |
| Popis: | Ammonia oxidising microorganisms (AOM) play an important role in ammonia removal in wastewater treatment works (WWTW) including rotating biological contactors (RBCs). Environmental factors within RBCs are known to impact the performance of key AOM, such that only some operational RBCs have shown ability for elevated ammonia removal. In this work, long-term treatment performance of seven full-scale RBC systems along with the structure and abundance of the ammonia oxidising bacteria (AOB) and archaea (AOA) communities within microbial biofilms were examined. Long term data showed the dominance of AOB in most RBCs, although two RBCs had demonstrable shift toward an AOA dominated AOM community. Next Generation Sequencing of the 16S rRNA gene revealed diverse evolutionary ancestry of AOB from RBC biofilms while nitrite-oxidising bacteria (NOBs) were similar to reference databases. AOA were more abundant in the biofilms subject to lower organic loading and higher oxygen concentration found at the distal end of RBC systems. Modelling revealed a distinct nitrogen cycling community present within high performing RBCs, linked to efficient control of RBC process variables (retention time, organic loading and oxygen concentration). We present a novel template for enhancing the resilience of RBC systems through microbial community analysis which can guide future strategies for more effective ammonia removal. To best of the author’s knowledge, this is the first comparative study reporting the use of next generation sequencing data on microbial biofilms from RBCs to inform effluent quality of small WWTW. Keywords: Biofilm, Rotating biological contactor, Nitrification, Wastewater treatment, Ammonia oxidising bacteria, Ammonia oxidising archaea |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect