Simian foamy virus type 1 is a retrovirus which encodes a transcriptional transactivator
| Autor: | Karen E. S. Shaw, Peter A. Barry, Elissa Pratt-Lowe, Ayalew Mergia, Paul A. Luciw |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Genes
Viral Transcription Genetic viruses Immunology Molecular Sequence Data Transfection Virus Replication Microbiology Virus Cell Line Transactivation Retrovirus Virology Sequence Homology Nucleic Acid Animals Humans Cloning Molecular Spumavirus Gene Repetitive Sequences Nucleic Acid biology Base Sequence Nucleic Acid Hybridization RNA Probes Human foamy virus biology.organism_classification Molecular biology Long terminal repeat Retroviridae Viral replication Insect Science Trans-Activators Plasmids Research Article |
| Zdroj: | Journal of virology. 64(8) |
| ISSN: | 0022-538X |
| Popis: | Simian foamy viruses, members of the spumavirus subfamily of retroviruses, are found in a variety of nonhuman primates and, as yet, remain to be characterized with respect to genetic structure and regulation of viral gene expression. The genome of simian foamy virus type 1 (SFV-1), an isolate from rhesus macaques, has been molecularly cloned, and the role of the viral long terminal repeat (LTR) in transcriptional control has been investigated. The SFV-1 LTR is 1,621 base pairs long, and sequence comparisons with human foamy virus revealed a pattern of clustered homology. A cap site in the LTR was identified by analysis of SFV-1 transcripts in infected cells. Transient expression assays in cell lines representing several species and different cell types showed that the SFV-1 LTR has low basal activity in uninfected cells, whereas LTR-directed expression is greatly increased in cells infected with SFV-1. This transactivation is mediated by a mechanism involving increases in steady-state levels of viral transcripts. Thus, the SFV-1 genome encodes a transactivator that functions on the LTR at the transcriptional level. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|