Creatine supplementation impairs airway inflammation in an experimental model of asthma involving P2 × 7 receptor
| Autor: | Ana Paula Ligeiro Oliveira, Robson Coutinho-Silva, Monique Garcia, Timo Siepmann, Marco Idzko, Jefferson Comin Jonco Aquino-Junior, Alana Santos-Dias, André Luis Lacerda Bachi, Nicole Cristine Rigonato-Oliveira, Sergio Cesar Ferreira, Francine Maria de Almeida, Rodolfo de Paula Vieira, Luiz Eduardo Baggio Savio, Manoel Carneiro Oliveira-Junior, Tobias Müller, Adilson Santos Andrade-Souza |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Immunology Biology Creatine Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Animals Humans Immunology and Allergy Receptor Whole blood Asthma Mice Inbred BALB C Pneumonia Purinergic signalling medicine.disease Disease Models Animal 030104 developmental biology Endocrinology chemistry Dietary Supplements Receptors Purinergic P2X7 Lymph Airway Adenosine triphosphate 030215 immunology |
| Zdroj: | European Journal of Immunology. 49:928-939 |
| ISSN: | 1521-4141 0014-2980 |
| DOI: | 10.1002/eji.201847657 |
| Popis: | Creatine (Cr) is a substrate for adenosine triphosphate synthesis, and it is the most used dietary supplement among professional and recreative athletes and sportsmen. Creatine supplementation may increase allergic airway response, but the cellular and molecular mechanisms are unknown. We used murine model of OVA-induced chronic asthma and showed that Cr supplementation increased total proteins, ATP level, lymphocytes, macrophages, and IL-5 levels in BALF, as well as IL-5 in the supernatant of re-stimulated mediastinal lymph nodes. IL-5 and IL-13 expression by epithelial cells and by peribronchial leukocytes were increased by Cr. Cr augmented the expression of P2 × 7 receptor by peribronchial leukocytes and by epithelial cells, and increased the accumulation of eosinophils in peribronchial space and of collagen fibers in airway wall. In human cells, while Cr induced a release of ATP, IL-6, and IL-8 from BEAS-2B cells, whole blood cells, such as eosinophils, and CD4+ T cells, P2 × 7 receptor inhibitor (A740003) reduced such effects, as denoted by reduced levels of ATP, IL-6, and IL-8. Therefore, Cr supplementation worsened asthma pathology due to activation of airway epithelial cells and peribronchial leukocytes, involving purinergic signaling. |
| Databáze: | OpenAIRE |
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