Oxygen-resistant multipotent embryonic carcinoma cell lines exhibit antimutator phenotypes
| Autor: | Mitchell S. Turker, George M. Martin, Charles E. Ogburn, Terrance J. Kavanagh, Annette C. Smith, Ken Ichiro Fukuchi, Christine M. Disteche |
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| Rok vydání: | 1994 |
| Předmět: |
Male
Hypoxanthine Phosphoribosyltransferase Mutation rate Adenine Phosphoribosyltransferase Cell Separation Biology Mice Tumor Cells Cultured Genetics Carcinoma medicine Animals Mice Inbred C3H Karyotype Cell Biology General Medicine Embryo Mammalian medicine.disease Embryonic stem cell Molecular biology Phenotype Oxygen Gamma Rays Cell culture Hemizygote Karyotyping Mutation Feasibility Studies Stem cell |
| Zdroj: | Somatic Cell and Molecular Genetics. 20:361-370 |
| ISSN: | 1572-9931 0740-7750 |
| DOI: | 10.1007/bf02257453 |
| Popis: | Stable, oxygen-resistant cell lines (O2R) were isolated from P19 and P19H22 (APRT hemizygote) mouse embryonic carcinoma cells by serial exposures of increasing durations to 95% O2. Neurally differentiated progeny were also oxygen-resistant. P19O2R exhibited reduced oxygen-mediated micronucleation and a 10- to 20-fold reduction of the forward mutation rate at the HPRT locus in 20% O2. P19H22O2R cells showed reduced frequencies of colonies resistant to 2,6-diaminopurine. The modal karyotype of P19O2R was identical to that of a nonmodal karyotype present in the parental line [39,X,-Y, add(14)]. There was no evidence of enhanced resistance to ionizing radiation. We conclude that this general approach, when applied to pluripotent embryonic stem cells, has the potential to lead to the synthesis of antimutator strains of mice. |
| Databáze: | OpenAIRE |
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