gammadelta T cells increase with gastric mucosal interleukin (IL)-7, IL-1beta, and Helicobacter pylori urease specific immunoglobulin levels via CCR2 upregulation in Helicobacter pylori gastritis
| Autor: | Hidemi Takahashi, Tetsuro Hiratsuka, Kazushi Ohashi, Kenji Suzuki, Shunji Kato, Seiji Futagami, Masanori Kusunoki, Choitsu Sakamoto, Taku Tsukui, Katya Gudis, Ken Wada, Masumi Shimizu, Kazumasa Miyake |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Receptors CCR2 medicine.medical_treatment T cell Interleukin-1beta Biology Helicobacter Infections T-Lymphocyte Subsets medicine Gastric mucosa Humans Cells Cultured Aged Aged 80 and over Lamina propria Hepatology Helicobacter pylori Interleukin-7 Gastroenterology Interleukin hemic and immune systems Receptors Antigen T-Cell gamma-delta Middle Aged biology.organism_classification Anti-Ulcer Agents Urease Anti-Bacterial Agents Immunoglobulin A Chemotaxis Leukocyte Cytokine medicine.anatomical_structure Gastric Mucosa Gastritis Immunoglobulin G Immunology Leukocyte Common Antigens Female Receptors Chemokine medicine.symptom CC chemokine receptors |
| Zdroj: | Journal of gastroenterology and hepatology. 21(1 Pt 1) |
| ISSN: | 0815-9319 |
| Popis: | BACKGROUND AND AIMS The purpose of this study was to investigate possible factors that could impact on gammadelta T cell accumulation in the gastric mucosa. METHOD Subjects were 22 Helicobacter pylori (H. pylori)-free and 75 H. pylori-infected mucosa biopsies classified into grades I approximately III gastritis as per our previous study. The number of gammadelta- and 45 RO-positive T cells were determined by immunostaining. Gastric mucosal anti-H. pylori urease specific antibodies and interleukin (IL)-1beta, IL-2, 4, 7, 10 and IL-12 levels were assayed by enzyme-linked immunosorbent assay (ELISA). CC chemokine receptor 2 (CCR2) expression levels, migration, and cytokine production in gammadelta T cells stimulated by H. pylori urease were also evaluated. RESULTS The gammadelta T cell count was significantly higher in grade III gastritis which exhibits strong immunoglobulin (Ig)A and IgG responses to H. pylori urease with lymphoid follicles than in other groups. gammadelta T cell count was significantly correlated with IL-1beta and interleukin-7 (IL-7) levels in the gastric mucosa. H. pylori urease immunoreactivity was detected in lamina propria of grade III gastritis, along with many gammadelta T cells. After H. pylori eradication therapy, the gammadelta T cell count in grade III gastritis significantly decreased. H. pylori urease stimulated significant increases in CCR2 expression levels, although to a lesser degree than those induced by IL-7 stimulation in both peripheral and mucosal gammadelta T cells. Interferon (IFN)-gamma and IL-10 production was also stimulated by H. pylori urease in peripheral gammadelta T cells. CONCLUSIONS Gastric mucosal increases in IL-7 and IL-1beta closely corresponded to the accumulation of gammadelta T cells in gastric mucosa. An association was also seen between gammadelta T cell accumulation and H. pylori urease-specific Ig levels. |
| Databáze: | OpenAIRE |
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