Intercellular adhesion molecule-1 expression and serum levels as markers of pre-clinical atherosclerosis in polycystic ovary syndrome

Autor: Hala G. Abomandour, Amal S. El-Shal, Mohammad Samir Badr, Maha A. Fathy, Nearmeen M. Rashad, Mohamed el sayed Rafeek, Mustafa taha Abdelfattah Sharaf el din, Ayman E. Ali, Amr Mostafa Kamel Aboelfath, Mohammed S. Yousef
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Adult
medicine.medical_specialty
endocrine system diseases
Gene Expression
030204 cardiovascular system & hematology
Cardiovascular
lcsh:Gynecology and obstetrics
Impaired glucose tolerance
Pathogenesis
Young Adult
03 medical and health sciences
0302 clinical medicine
Insulin resistance
Enzyme-linked immunosorbent assay
Internal medicine
Type 2 diabetes mellitus
medicine
Humans
RNA
Messenger
Polycystic ovary syndrome
lcsh:RG1-991
030219 obstetrics & reproductive medicine
business.industry
Research
Hyperandrogenism
Case-control study
Obstetrics and Gynecology
Type 2 Diabetes Mellitus
nutritional and metabolic diseases
Intercellular adhesion molecule-1
Middle Aged
Atherosclerosis
medicine.disease
Polycystic ovary
Real-time polymerase chain reaction
Endocrinology
ROC Curve
Oncology
Case-Control Studies
Female
business
Biomarkers
Zdroj: Journal of Ovarian Research, Vol 12, Iss 1, Pp 1-12 (2019)
Journal of Ovarian Research
ISSN: 1757-2215
Popis: Background Polycystic ovary syndrome (PCOS) is a common reproductive endocrine disorder characterized by obesity, hyperandrogenism, and insulin resistance. Intercellular adhesion molecule-1 (ICAM-1) is a proinflammatory and proatherogenic cytokine which is associated with atherosclerosis, insulin resistance, and cardiovascular disease (CVD). The pathogenesis of PCOS is not precisely known. Thus, the purpose of this study was to investigate the potential role of ICAM-1 expression and serum ICAM-1 concentrations in pathogenesis of PCOS. Moreover, we aimed to evaluate the possible relationship between ICAM-1 gene expression with carotid intima-media thickness as well as clinic-morphological features of PCOS. Methods This case control study enrolled 180 patients with PCOS and 120 controls groups and they were stratified according to their fasting plasma glucose (FPG) into three subgroups; normal glucose tolerance (NGT) [n = 75], those with impaired glucose tolerance (IGT) [n = 65], and 40 patients with type 2 diabetes mellitus (T2DM). Circulating ICAM-1 expression levels were determined by real time polymerase chain reaction (RT-PCR). Serum ICAM-1 concentrations were measured using enzyme-linked immunosorbent assay (ELISA). Results Our results revealed that PCOS patients had higher values of ICAM-1expression and serum levels. Among PCOS patients, T2DM patients had the highest values of ICAM-1 expression and serum levels compared to IGT and NGT subgroups. The ICAM-1 expression and serum levels were significantly positive correlated with cardiovascular risk and PCOS phenotypes. Linear regression test showed that HOMA-IR was the main predictors of serum ICAM-1 levels in PCOS. Receiver operating characteristic curve (ROC) analysis revealed that, the power of ICAM-1 expression levels was higher than serum ICAM-1 in diagnosis of PCOS and in differentiating T2DM from IGT and NGT subgroups. Interestingly, combination of both ICAM-1 expression and serum levels improved the diagnostic role of serum ICAM-1. Conclusion ICAM-1 expression and serum levels were higher in women with PCOS compared to control group also, there was a strong independent association between higher ICAM-1 expression and serum levels with cardiovascular risks in PCOS group.
Databáze: OpenAIRE
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