Stimulation of renal microvascular development under organotypic culture conditions
| Autor: | A Schmidbauer, Sabine Kloth, W W Minuth, M Kubitza, C Ebenbeck, W. Röckl |
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| Rok vydání: | 1995 |
| Předmět: |
Kidney Cortex
Endothelium Basic fibroblast growth factor Kidney Biochemistry Cholangiocyte Immunoenzyme Techniques Extracellular matrix chemistry.chemical_compound Organ Culture Techniques Perfusion Culture Genetics medicine Animals Aldosterone Molecular Biology Cholecalciferol Microcirculation Antibodies Monoclonal Culture Media Cell biology Perfusion Vascular endothelial growth factor medicine.anatomical_structure Animals Newborn chemistry Fibroblast Growth Factor 2 Endothelium Vascular Rabbits Biotechnology |
| Zdroj: | The FASEB Journal. 9:963-967 |
| ISSN: | 1530-6860 0892-6638 |
| DOI: | 10.1096/fasebj.9.10.7615165 |
| Popis: | The development of the renal vascular system requires the coordinated action of soluble morphogenic factors and specific extracellular matrix components. Despite intensive research it remains unknown whether the humoral or the environmental component is more important in the development of renal microvessels. The prolonged serum-free culture of embryonic kidney cortex explants was achieved by means of a newly developed perfusion culture system. This system made the investigation of renal vascular development under defined organotypic conditions possible. Thus, growth factors such as vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and hormones (aldosterone, vitamin D3) could be applied without the interference with serum components. Medium supplementation with VEGF or aldosterone in combination with vitamin D3 resulted in the coordinated proliferation of endothelial cells in the explant. A well-developed collecting duct epithelium and numerous tubular structures were always observed. In contrast, only a uniform cell layer was found between fibrous organ capsule and the collecting duct epithelium after bFGF application, but neither tubular structures nor endothelial cells. Thus, the experiments indicate that bFGF alone has no stimulating effect on the growth of the renal microvasculature under perfusion culture conditions. |
| Databáze: | OpenAIRE |
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