Evaluation of protein A and protein G as indicator system in an ELISA for detecting antibodies in mink to Pseudomonas aeruginosa
| Autor: | Esteban Rivera, M. Jackert-Jernberger, T. Mejerland, Karl-Axel Karlsson |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
G protein Immunoprecipitation Enzyme-Linked Immunosorbent Assay Microbiology biology.animal Animals Pseudomonas Infections Mink Staphylococcal Protein A General Veterinary biology Binding protein General Medicine Virology Molecular biology Antibodies Bacterial Bacterial Vaccines Pseudomonas aeruginosa biology.protein Female Protein G Antibody Protein A Peroxidase |
| Zdroj: | Veterinary microbiology. 42(4) |
| ISSN: | 0378-1135 |
| Popis: | A modified, indirect enzyme-linked immunosorbent assay (ELISA) was developed and applied in the detection of mink antibodies to Pseudomonas aeruginosa. In this assay, peroxidase conjugated protein A and protein G were evaluated as indicator systems for detecting antigen-antibody complexes. It was found that protein A has a strong affinity for mink immunoglobulins. In contrast, protein G showed no such affinity. The affinity of protein A for mink immunoglobulins was further demonstrated by immunoprecipitation assays. |
| Databáze: | OpenAIRE |
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