Immunodot Assay of Plasmodium falciparum Sporozoites in Mosquitoes Using a Direct Binding Membrane System
| Autor: | Gary W. Long, John J. Oprandy, Yupin Charoenvit |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
medicine.drug_class
Immunoblotting Plasmodium falciparum Protozoan Proteins Antibodies Protozoan Antigens Protozoan Biology Monoclonal antibody chemistry.chemical_compound Antigen Virology Anopheles parasitic diseases medicine Animals Sodium dodecyl sulfate Parasite Egg Count Anopheles stephensi medicine.diagnostic_test Membranes Artificial biology.organism_classification Molecular biology Circumsporozoite protein Infectious Diseases chemistry Immunoassay biology.protein Parasitology Antibody Protein Binding |
| Zdroj: | The American Journal of Tropical Medicine and Hygiene. 43:597-601 |
| ISSN: | 1476-1645 0002-9637 |
| DOI: | 10.4269/ajtmh.1990.43.597 |
| Popis: | We describe a membrane based immunodot assay for the detection of Plasmodium falciparum sporozoites mixed with mosquitoes. A crude sodium dodecyl sulfate extract of mosquitoes and sporozoites is passed through a bi-layered membrane system, the top layer being a polyvinyldiene difluoride hydrophilic pre-filter which screens out debris but allows the passage of antigen. Sporozoite, as well as mosquito, proteins are bound to the hydrophobic membrane below. This membrane was probed with a monoclonal antibody to the repeat region of the P. falciparum circumsporozoite protein, a peroxidase labeled second antibody and a tetramethyl-benzidine substrate. The method detects as few as 10 sporozoites/mosquito or 100 sporozoites in a pool of 10. |
| Databáze: | OpenAIRE |
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