No evidence that vitellogenin protein expression is induced in marine mussels after exposure to an estrogenic chemical
| Autor: | Angel P. Diz, Laura Emilia Fernández-González, Soledad Muniategui-Lorenzo, Ricardo Beiras, Paula Sánchez-Marín, Noche Gloria Grueiro |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Proteomics Environmental Engineering Gonad 010504 meteorology & atmospheric sciences Medio Marino y Protección Ambiental Endocrine disruption Zoology 010501 environmental sciences Endocrine Disruptors Ethinyl Estradiol 01 natural sciences Protein expression Vitellogenin Vitellogenins Tandem Mass Spectrometry Centro Oceanográfico de Vigo medicine Environmental Chemistry Endocrine system Animals Waste Management and Disposal 0105 earth and related environmental sciences Mollusks biology 17α-ethinylestradiol fungi Marine invertebrates Mussel Biomarker biology.organism_classification Pollution Mytilus medicine.anatomical_structure biology.protein Biomarker (medicine) Female Water Pollutants Chemical Chromatography Liquid |
| Zdroj: | The Science of the total environment. 721 |
| ISSN: | 1879-1026 |
| Popis: | A wide variety of endocrine disrupting chemicals reach the marine environment and can cause harmful effects in different marine organisms. Vitellogenin (Vtg), the egg-yolk precursor, is a commonly used endocrine disruption biomarker in fish and more recently in marine invertebrates under the assumption of high expected similarities in the endocrine system of vertebrates and invertebrates. However, this assumption has been recently questioned. The results from previous studies focused on bivalve molluscs showed that Vtg induction could be misleading because of the use of either non-robust or indirect techniques to measure Vtg. In this study, mussels (Mytilus galloprovincialis) were exposed to either 10 or 100 ng/L of the synthetic hormone 17α-ethinylestradiol (EE2) at different exposure times (4 and 24 days) and under different feeding regimes (representing different energy balances), and Vtg levels in both male and female mussel gonads were quantified by label free shotgun LC-MS/MS proteomic analysis. Vtg protein was not detected in male gonads. In female gonads, Vtg levels were not significantly affected by EE2 at any exposure time or EE2 concentration tested, whereas a significant correlation was found between the degree of maturation of the gonad and Vtg levels in females. Results obtained in the present study critically question the use of Vtg as a biomarker of endocrine disruption in marine mussels, and show that the degree of maturation of the gonad can be an important confounding factor in the attempts to evaluate estrogenic effects through Vtg measurement in mussel gonads. |
| Databáze: | OpenAIRE |
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