Mitochondrial hyperpolarization and cytochrome-c release in microwave-exposed MCF-7 cells
| Autor: | Ayşe G. Canseven, Handan Kayhan, Nesrin Seyhan, Bahriye Sirav, Mehmet Zahid Tuysuz, Meric Arda Esmekaya |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Physiology Biophysics Apoptosis Mitochondrion Radiation Dosage Mitochondrial apoptosis-induced channel Gene Expression Regulation Enzymologic 03 medical and health sciences 0302 clinical medicine Humans Microwaves Membrane Potential Mitochondrial Membrane potential biology Chemistry Cytochrome c Cytochromes c Dose-Response Relationship Radiation General Medicine Hyperpolarization (biology) Molecular biology Mitochondria Staining Dose–response relationship 030104 developmental biology 030220 oncology & carcinogenesis MCF-7 Cells biology.protein |
| Zdroj: | General physiology and biophysics. 36:211-218 |
| ISSN: | 1338-4325 |
| Popis: | This study examines the effects of a 2.1-GHz WCDMA-modulated microwave (MW) radiation on apoptotic activity and mitochondrial membrane potential (Delta Psi(m)) in MCF-7 cells. The cells were exposed to the MW at a specific absorption rate (SAR) of 0.528 W/kg for 4 or 24 h. The antiproliferative effect of MW exposure was determined by the MTT test. Cytochrome-c and p53 levels were determined by an ELISA method. The relative Delta Psi(m) was analysed by JC-1 staining using flow cytometer. Apoptotic rate of the cells was measured by Annexin-V-FITC staining. All assays were performed after certain time of incubations (15 min-4 h) following MW exposure. MW-exposed cells showed a significant decrease in viability when compared to unexposed cells. A significantly larger decrease was observed after longer exposure. The percentage of apoptotic cells, amount of cytochrome-c, and relative Delta Psi(m) were significantly higher in MW-exposed cells. The percent of apoptotic cells and relative Delta Psi(m). in 24 h MW-exposed group was significantly higher than those in 4 h MW-exposed group. However, no significant change was observed in p53 levels. These results demonstrated that exposure to 2.1-GHz WCDMA-modulated MW radiation caused hyperpolarization of mitochondria that in turn induced apoptosis in MCF-7 cells. |
| Databáze: | OpenAIRE |
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