Control of Drosophila Growth and Survival by the Lipid Droplet-Associated Protein CG9186/Sturkopf
| Autor: | Alisa Gahlen, Thomas O. Eichmann, Mathias Beller, Gereon Poschmann, Kerstin Maas-Enriquez, Michael Werthebach, Fiona A. Stewart, Kai Stühler, Andrea Droste, Irfan Akhtar, Tabea Mettler-Altmann |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Mutant Nutrient sensing Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Lipid droplet Organelle Animals Body Size Drosophila Proteins Insulin lcsh:QH301-705.5 Gene Triglycerides fungi Gene Expression Regulation Developmental Lipid Droplets Phenotype Diet Cell biology Juvenile Hormones Insulin receptor Drosophila melanogaster 030104 developmental biology lcsh:Biology (General) Mutation Juvenile hormone biology.protein Carboxylic Ester Hydrolases Locomotion 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Cell Reports, Vol 26, Iss 13, Pp 3726-3740.e7 (2019) |
| ISSN: | 2211-1247 |
| DOI: | 10.1016/j.celrep.2019.02.110 |
| Popis: | Summary: Lipid droplets (LDs) are the universal cellular storage organelles for esterified neutral lipids. The increasing number of characterized LD-associated proteins attained LDs with hitherto unexpected functions on top of their classical role as energy depot. Here, we characterize the LD-associated protein CG9186 of Drosophila by a CRISPR/Cas9-derived mutant fly line. While the mutant flies only showed a mild triacylglycerol storage phenotype, they were highly protected from desiccation stress, likely linked to a reduced locomotor activity and altered cuticular hydrocarbons. Both parameters depend on juvenile hormone (JH) signaling. Together with an observed interaction between CG9186 and JH-degrading enzymes, our results suggest that CG9186 participates in endocrine physiology regulation. In support of this hypothesis, CG9186 mutant flies show an altered expression of JH target genes and fail to adjust their developmental rate to dietary yeast-to-sugar ratio changes. Our results thus link LDs to organismic physiology regulation. : Werthebach et al. introduce the lipid droplet-associated protein CG9186/sturkopf as a component of organismic physiology regulation, which affects insulin and juvenile hormone signaling activity. Keywords: lipid droplets, physiology, Drosophila, larval developmental rate, nutrient sensing, juvenile hormone, LDAH, c2orf43, desiccation, insulin signaling |
| Databáze: | OpenAIRE |
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