Biological Activity of Brain-derived Neurotrophic Factor with Mismatched Disulfide Linkages Produced byEscherichia coli
| Autor: | Norio Shimizu, Tadashi Ishibashi, Shinichi Fukuzono, Tomoko Takeshita, Kyungmoon Park, Kiyoshi Fujimori |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Recombinant Fusion Proteins
Nerve Tissue Proteins Chick Embryo medicine.disease_cause Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry law.invention Neurotrophic factors law Complementary DNA Escherichia coli medicine Animals Disulfides Cloning Molecular Molecular Biology Neurons Brain-derived neurotrophic factor Expression vector Chemistry Brain-Derived Neurotrophic Factor Organic Chemistry Biological activity General Medicine In vitro Rats nervous system Recombinant DNA Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 60:971-974 |
| ISSN: | 1347-6947 0916-8451 |
| Popis: | E. coli cells harboring an expression plasmid with a brain-derived neurotrophic factor (BDNF) gene obtained from rat BDNF cDNA were sonicated and centrifuged to obtain a precipitate containing BDNF. Ten different proteins of BDNF were purified from the precipitate and the three disulfide linkages of six proteins were identified. Those disulfide structures were different from that of authentic BDNF and the biological activities of BDNF with mismatched disulfide linkages (EC50 of 2 to 15 ng/ml) were much lower than that of authentic BDNF (EC50 of 30 pg/ml). The BDNF with mismatched disulfide linkages inhibited the biological activity of authentic BDNF. |
| Databáze: | OpenAIRE |
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