Development and evaluation of a new interferon-gamma release assay for the diagnosis of tuberculosis infection in HIV-infected individuals in China

Autor:	Ruiling Fu, Nicholas Kushner, Zhongfang Zhao, Yichen Lu, Binying Ji, Mai Yang, Zeping Wei, Neal Touzjian, Shu Li, Jian Wang, Liang Yu, Pingzheng Mo, Xi-En Gui, Amie J. Strong
Rok vydání:	2015
Předmět:	Microbiology (medical) Adult Male China Tuberculosis Concordance Population Human immunodeficiency virus (HIV) Interferon gamma release assay Tuberculin HIV Infections Disease medicine.disease_cause Mycobacterium tuberculosis medicine Humans education education.field_of_study Bacteriological Techniques General Immunology and Microbiology biology business.industry Tuberculin Test General Medicine Middle Aged bacterial infections and mycoses biology.organism_classification medicine.disease Virology CD4 Lymphocyte Count Infectious Diseases Immunology Female business Interferon-gamma Release Tests
Zdroj:	Infectious diseases (London, England). 47(4)
ISSN:	2374-4243
Popis:	Human immunodeficiency virus (HIV)-infected individuals are at high risk of contracting tuberculosis (TB) disease, and current methods for diagnosing TB infection are less effective in this population. We developed and evaluated a new interferon-gamma release assay (IGRA), named A.TB, in HIV-infected individuals, with and without active TB, in a setting of high TB burden and low HIV prevalence.A total of 255 subjects were divided into 3 groups according to their HIV and TB status: HIV+ without active TB (n = 123), HIV+/TB+ (n = 79), and HIV-/TB+ (n = 65). The A.TB assay was performed in parallel with the QuantiFERON-TB Gold In-Tube (QFT-GIT) and tuberculin skin test (TST).The positive rate was 59.3% (n = 123) by A.TB and 53.8% (n = 106) by QFT-GIT. We observed a strong concordance of 81.2% (k = 0.612) between the two IGRAs. The QFT-GIT results were affected by low CD4(+) cell count (p = 0.013), while A.TB results were not. A.TB was also performed in patients with active TB (n = 65) and patients with active TB and HIV co-infection (n = 79). The sensitivity of A.TB in these groups was 80.0% and 81.0%, respectively.The A.TB results were not affected by low CD4(+) cell count in the co-infected cohort. With further evaluation, A.TB may prove to be a valuable tool for diagnosing TB in HIV-infected patients.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52154b2a356d78ee9e41d4085eb4b44e https://pubmed.ncbi.nlm.nih.gov/25712792 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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