MicroRNA miR-9 Modifies Motor Neuron Columns by a Tuning Regulation of FoxP1 Levels in Developing Spinal Cords
| Autor: | Tao Sun, Andrew Pollock, Janet Hong, Gaizka Otaegi |
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| Rok vydání: | 2011 |
| Předmět: |
Blotting
Western Regulator Chick Embryo Biology Article microRNA medicine Animals Hox gene Transcription factor In Situ Hybridization Motor Neurons Regulation of gene expression Analysis of Variance Gene knockdown General Neuroscience Gene Expression Regulation Developmental Forkhead Transcription Factors Motor neuron Spinal cord Immunohistochemistry Repressor Proteins MicroRNAs medicine.anatomical_structure Spinal Cord nervous system Neuroscience |
| Zdroj: | The Journal of Neuroscience. 31:809-818 |
| ISSN: | 1529-2401 0270-6474 |
| DOI: | 10.1523/jneurosci.4330-10.2011 |
| Popis: | The precise organization of motor neuron subtypes in a columnar pattern in developing spinal cords is controlled by cross-interactions of multiple transcription factors and segmental expressions of Hox genes and their accessory proteins. Accurate expression levels and domains of these regulators are essential for organizing spinal motor neuron columns and axonal projections to target muscles. Here, we show that microRNA miR-9 is transiently expressed in a motor neuron subtype and displays overlapping expression with its target gene FoxP1. Overexpression or knockdown of miR-9 alters motor neuron subtypes, switches columnar identities, and changes axonal innervations in developing chick spinal cords. miR-9 modifies spinal columnar organization by specifically regulating FoxP1 protein levels, which in turn determine distinct motor neuron subtypes. Our findings demonstrate that miR-9 is an essential regulator of motor neuron specification and columnar formation. Moreover, the overlapping expression of miR-9 and its target FoxP1 further illuminates the importance of fine-tuning regulation by microRNAs in motor neuron development. |
| Databáze: | OpenAIRE |
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