Melanoma-associated B cells are distinct from peripheral blood-derived B cells, and may serve as a source of tumor-targeting antibodies
| Autor: | Theresa A Alexander, Steven A. Rosenberg, Daniel Abate-Daga, Paul F. Robbins, Evgeny Arons, Richard A. Morgan, Mitchell Ho |
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| Rok vydání: | 2013 |
| Předmět: |
Pharmacology
CD86 Cancer Research Cell type Lymphocyte T cell Immunology Biology Molecular biology medicine.anatomical_structure Oncology Poster Presentation medicine Activation-induced (cytidine) deaminase biology.protein Molecular Medicine Immunology and Allergy Antibody Antigen-presenting cell B cell |
| Zdroj: | Journal for Immunotherapy of Cancer |
| ISSN: | 2051-1426 |
| DOI: | 10.1186/2051-1426-1-s1-p180 |
| Popis: | The tumor microenvironment contains a complex mix of cell types that can modulate the anti-tumor immune response. In the present study we performed a characterization of tumor-associated B cells, aimed at understanding the biological implications of B cell infiltration in metastases of melanoma patients. We performed a comparative analysis between B cells present in peripheral blood (PBMC) of metastatic melanoma patients and those present in single cell suspensions prepared from metastatic lesions (FrTu) of the same patients. Flow cytometry analysis of these populations showed that the mean B-to-T cell ratio was 5-fold higher in FrTu than in PBMC (p= 0.004, n= 14). Spectratyping of immunoglobulin genes present in FrTu-B revealed a profile that is compatible with an oligoclonal population, as opposed to the polyclonal distribution observed in PBMC-B. Next, we analyzed the expression of 511 genes related to immune cell function in PBMC-B and FrTu-B cells of seven patients. Using a digital platform for direct quantitation of mRNA molecules, we identified forty genes that were differentially expressed (fold change>2, p |
| Databáze: | OpenAIRE |
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