A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions
| Autor: | Marina C. M. Martins, Camila Caldana, Viviane C. H. da Silva, Elias F Araujo, Carolina Cassano Monte-Bello, Viviane Celente, Valéria Mafra |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cost-Benefit Analysis Microorganism General Chemical Engineering 01 natural sciences General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Hydroponics Arabidopsis Arabidopsis thaliana biology General Immunology and Microbiology Setaria viridis General Neuroscience fungi food and beverages Contamination biology.organism_classification Horticulture 030104 developmental biology Homogeneous Shoot Environmental Sciences 010606 plant biology & botany |
| Zdroj: | J Vis Exp |
| ISSN: | 1940-087X |
| Popis: | A wide range of studies in plant biology are performed using hydroponic cultures. In this work, an in vitro hydroponic growth system designed for assessing plant responses to chemicals and other substances of interest is presented. This system is highly efficient in obtaining homogeneous and healthy seedlings of the C(3) and C(4) model species Arabidopsis thaliana and Setaria viridis, respectively. The sterile cultivation avoids algae and microorganism contamination, which are known limiting factors for plant normal growth and development in hydroponics. In addition, this system is scalable, enabling the harvest of plant material on a large scale with minor mechanical damage, as well as the harvest of individual parts of a plant if desired. A detailed protocol demonstrating that this system has an easy and low-cost assembly, as it uses pipette racks as the main platform for growing plants, is provided. The feasibility of this system was validated using Arabidopsis seedlings to assess the effect of the drug AZD-8055, a chemical inhibitor of the target of rapamycin (TOR) kinase. TOR inhibition was efficiently detected as early as 30 min after an AZD-8055 treatment in roots and shoots. Furthermore, AZD-8055-treated plants displayed the expected starch-excess phenotype. We proposed this hydroponic system as an ideal method for plant researchers aiming to monitor the action of plant inducers or inhibitors, as well as to assess metabolic fluxes using isotope-labeling compounds which, in general, requires the use of expensive reagents. |
| Databáze: | OpenAIRE |
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