DNA immunization with pgp3 gene of Chlamydia trachomatis inhibits the spread of chlamydial infection from the lower to the upper genital tract in C3H/HeN mice
| Autor: | Giulio Ratti, Roberto Cevenini, Vittorio Sambri, Elisa Storni, Lorenzo Giacani, Manuela Donati, Maurizio Comanducci, Korinne Di Leo |
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| Rok vydání: | 2003 |
| Předmět: |
Serotype
Ratón Genetic Vectors Chlamydia trachomatis medicine.disease_cause Salpingitis Microbiology Mice Bacterial Proteins Vaccines DNA medicine Animals Chlamydiaceae Antigens Bacterial Mice Inbred C3H General Veterinary General Immunology and Microbiology biology Public Health Environmental and Occupational Health Immunotherapy Active Vaginosis Bacterial Chlamydia Infections biochemical phenomena metabolism and nutrition biology.organism_classification Virology Infectious Diseases Immunization Cell culture Chlamydiales Disease Progression biology.protein bacteria Molecular Medicine Female Antibody Pelvic Inflammatory Disease |
| Zdroj: | Vaccine. 21:1089-1093 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/s0264-410x(02)00631-x |
| Popis: | Chlamydia trachomatis pgp3 DNA immunized (no. 300) and non-immunized (no. 300) C3H/HeN mice were infected by vaginal inoculation with infectious C. trachomatis serotype D elementary bodies (EBs) and the spread of infection to the salpinges was assessed by cell culture isolation from tissue homogenates 7, 14, 21, 28, 35 and 42 days post-infection (p.i.). Overall, the pgp3-DNA immunization prevented salpinx infection in 94 (56%) mice, if compared with the 168 positive animals found among the non-immunized animals (P < 0.001). A group of negative control animals (i.e. mice immunized with plasmid DNA containing an irrelevant insert) was not protected, whereas all the mice of a positive immune control group (mice that had resolved a primary genital C. trachomatis infection) were resistant to re-infection. Pgp3 DNA immunization induced both humoral and mucosal anti-pgp3 antibodies. |
| Databáze: | OpenAIRE |
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