Serological and molecular evidence of coxiellosis and risk factors in sheep flocks in central-eastern Tunisia

Autor: Radhouane Gdoura, Imen Fendri, Mohamed Barkallah, Mariem Hmani, Michel Gautier, Yaakoub Gharbi, Zouhir Mallek
Přispěvatelé: Toxicology-Microbiology and Environmental Health Laboratory, Faculty of Sciences, Université de Sfax - University of Sfax, Veterinary Research center of Sfax, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Ministère de l’enseignement supérieur et de la recherche scientifique' and the ISESCO Organization.
Rok vydání: 2017
Předmět:
Veterinary medicine
prévention des maladies
Logistic regression
law.invention
Serology
0403 veterinary science
0302 clinical medicine
Hygiene
law
Pregnancy
Risk Factors
Prevalence
Immunology and Allergy
media_common
wether hoggs
santé animale
disease prevention
Vaccination
indirect elisa
04 agricultural and veterinary sciences
General Medicine
Abortion
Veterinary
Infectious Diseases
test elisa
facteur de risque
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Milk
risk factor
Coxiella burnetii
Bacterial Vaccines
Vagina
Female
Q Fever
DNA
Bacterial
Farms
Tunisia
mouton
040301 veterinary sciences
media_common.quotation_subject
030231 tropical medicine
Immunology
Sheep Diseases
Biology
Microbiology
Tunisie
03 medical and health sciences
Quarantine
coxiella
Animals
technique elisa
pcr en temps réel
Sheep
General Veterinary
animal health
Immune Sera
biology.organism_classification
Herd
coxiellosis
Flock
Zdroj: Comparative Immunology, Microbiology and Infectious Diseases
Comparative Immunology, Microbiology and Infectious Diseases, Elsevier, 2018, 57, pp.15-21. ⟨10.1016/j.cimid.2018.02.001⟩
ISSN: 1878-1667
0147-9571
DOI: 10.1016/j.cimid.2018.02.001⟩
Popis: In this study, we conducted an investigation to determine the true prevalence of coxiellosis in sheep in central-eastern Tunisia. A total of 492 veterinary samples taken from 110 flocks were screened for coxiellosis using IS1111-based real-time PCR assay. Sheep sera were tested using an indirect enzyme-linked immunosorbent assay. Based on molecular and serological results, the true adjusted animal and herd-level prevalence of coxiellosis were 11.8% and 20.21%, respectively. Bacterial excretion was observed in 17 flocks, and 19 females showed evidence of Coxiella burnetii shedding (100%). In addition, a statistically significant association was found between vaginal and milk shedding for sheep. Multivariable logistic regression analysis at the animal-population level indicated that strata and vaccination variables were found to be associated with coxiellosis. Besides, it was shown that this infection increased when the intensive farm was exposed to carnivores and when the cleaning practices were not respected, while it decreased when a suitable quarantine was introduced for any introduction of a new animal. Good hygiene and sanitation practices on-farm should be handled as strategies to deal with this zoonotic pathogen in herds.
Databáze: OpenAIRE
Externí odkaz: