Protective effects of anthocyanins on the ectonucleotidase activity in the impairment of memory induced by scopolamine in adult rats
| Autor: | Claudio A.M. Leal, Michele Melgarejo Rosa, Jessié Martins Gutierres, Victor C. Pimentel, Cinthia M. Mazzanti, Marilia Valvassori Rodrigues, Daniela Aymone Ribeiro, Fabiano B. Carvalho, Maria Rosa Chitolina Schetinger, Roselia Maria Spanevello, Juliano Marchi Vieira, Patricia C. Marisco, Maribel Antonello Rubin, Roberta Schmatz |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Stereochemistry Scopolamine Hippocampus Pharmacology Inhibitory postsynaptic potential Neuroprotection General Biochemistry Genetics and Molecular Biology Open field Anthocyanins Pharmacology Toxicology and Pharmaceutics(all) Adenosine deaminase Nucleotidases Memory Avoidance Learning medicine Animals Ectonucleotidase Rats Wistar General Pharmacology Toxicology and Pharmaceutics Chromatography High Pressure Liquid Cerebral Cortex Analysis of Variance Memory Disorders Ectonucleotidases L-Lactate Dehydrogenase biology Chemistry Biochemistry Genetics and Molecular Biology(all) Purinergic receptor General Medicine Rats medicine.anatomical_structure Cerebral cortex biology.protein Synaptosomes |
| Zdroj: | Life Sciences. 91(23-24):1221-1228 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/j.lfs.2012.09.013 |
| Popis: | Aims We investigated whether the treatment with anthocyanins prevents the scopolamine-induced memory deficits and whether ectonucleotidase activities and purine levels are altered in the cerebral cortex (CC) and hippocampus (HC) in this model of mnemonic deficit in rats. Main methods The animals were divided into 4 experimental groups: control (vehicle), anthocyanins (Antho), scopolamine (SCO), and scopolamine plus anthocyanins (SCO + Antho). After seven days of treatment, they were tested in the inhibitory avoidance task and open field test and submitted to euthanasia. The CC and the HC were collected for biochemical assays. The effect of treatment with Antho (200 mg kg − 1 , i.p.) was investigated in rats trained to a stable level of performance and post-treated with SCO (1 mg kg − 1 , i.p. 30 min after training). Key findings The treatment with SCO decreased the step-down latency in inhibitory avoidance task. Antho prevented the scopolamine-induced memory impairment and also the increase of NTPDase activity in the CC and HC. Furthermore, the treatment with anthocyanins prevents the decrease in 5′-nucleotidase activity and the increase in adenosine deaminase activity induced by SCO in HC. In addition, the treatment with Antho prevented the decrease in ATP levels induced by SCO in the CC and HC. Significance Our results show that scopolamine may affect purinergic enzymatic cascade or cause alterations in energy metabolism inducing loss of memory. In contrast Antho could reverse these changes, suggesting a neuroprotective effect of Antho on ectonucleotidase activities and neuronal energetic metabolism. |
| Databáze: | OpenAIRE |
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