Organization of trigeminocollicular connections and their relations to the sensory innervation of the eyelids in the rat
| Autor: | Frans VanderWerf, Gabrielle Pinganaud, Pierre Buisseret, Awa Ndiaye, Catherine Buisseret-Delmas |
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| Rok vydání: | 2002 |
| Předmět: |
Neurons
Afferent Pathways Brain Mapping Superior Colliculi General Neuroscience Superior colliculus Sensation Eyelids Colocalization Sensory system Anatomy Biology Trigeminal Nuclei Protein expression Rats Rats Sprague-Dawley Neuronal tracing medicine.anatomical_structure Afferent Neural Pathways medicine Animals Eyelid Proto-Oncogene Proteins c-fos Nucleus |
| Zdroj: | The Journal of Comparative Neurology. 448:373-387 |
| ISSN: | 1096-9861 0021-9967 |
| Popis: | Relationships between the trigeminal component of blinking and the superior colliculus (SC) were studied in rats. To localize primary afferent eyelid projections in the sensory trigeminal complex, neuronal tracing experiments were performed as well as analysis of c-Fos protein expression after supraorbital (SO) nerve stimulation. Labelled nerve fibers were found to enter ventrally within the ipsilateral sensory trigeminal complex. Labelled boutons were observed at the junction of the principal nucleus (5P) and the pars oralis (5o) and in the pars caudalis (5c). The c-Fos immunoreactivity was observed in neurons located in the ipsilateral ventral parts of 5P, 5o, and the pars interpolaris (5i) and bilaterally in 5c. Injections in 5P, 5o, 5i, and 5c resulted in anterogradely labelled fibers, with a contralateral preponderance, within the intermediate and deeper SC layers. Injections in 5P or 5o showed anterogradely labelled nerve fibers, profusely terminating in small patches in the medial and central portions of SC layer 4. Subsequently, dense labelling was found in the lateral portion of SC layers 4-7, without patch-like organization. Injections in SC showed retrogradely labelled neurons predominantly within the contralateral part of the sensory trigeminal complex (28% in 5P, 20% in 5o, 50% in 5i, and 2% in 5c). Colocalization of the retrograde tracer after SC injections and c-Fos immunoreactivity in neurons demonstrated that some 5P, 5o, and 5i neurons receive SO nerve inputs and project to SC. This implies that intermediate and deeper SC layers receive sensory information from the eyelids and may be directly involved in the regulation of eye-eyelid coordination. |
| Databáze: | OpenAIRE |
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